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Cell-free Kits - Overview

 

Cell-free Protein Synthesis Kits

Cell-free synthesis kit

Reaction
yield

Reaction
volume

Reaction
time (hrs)

Expression
template

Expression
mode

Protein classes expressed

RTS 100 E. coli HY Kit

20 μg

50 μl

1–4

Linear: 1, 3, 4
Vector: 6, 8

Batch

Standard soluble
Membrane*

RTS 500 ProteoMaster
E. coli  HY Kit

6 mg

1 ml

6–24

Vector: 6, 8

CECF

Standard soluble
Membrane*

RTS 9000 E. coli HY Kit 

50 mg

10 ml

6–24

Vector: 6, 8

CECF

Standard soluble
Membrane*

RTS 100 E. coli Disulfide Kit

80 μg

50 μl

6–24

Vector: 6, 8

CECF

Standard soluble, Disulfide-
bonded, (Fab antibodies)

RTS 500 E. coli Disulfide Kit 

2.5 mg

1 ml

6–24

Vector: 6, 8

CECF

Standard soluble, Disulfide-
bonded, (Fab antibodies)

RTS 100 Wheat Germ Kit

5 μg

50 μl

3

Linear: 2
Vector: 7

Batch

Complex and large, Standard
Membrane*

RTS 100 Wheat Germ CECF Kit

50 μg

50 μl

6–24

Linear: 2
Vector: 7

CECF

Complex and large, Standard
Membrane*

RTS 500 Wheat Germ CECF Kit 

1 mg

1 ml

6–24

Linear: 2
Vector: 7

CECF

Complex and large, Standard
Membrane*

RTS 100 Insect Membrane Kit

2 μg

50 μl

4

Linear: 1, 3, 4
Vector: 5, 6, 8

Batch

Complex and large, Standard
Membrane

    RTS: Rapid (cell-free) translation system   |   CECF: Continuous exchange cell-free expression   |   Batch: Expression in batch format   |   * using detergents

Linear Templates and Vectors for Cell-Free Protein Synthesis

No.

PCR generated linear template

Protein tag

1

RTS Linear Template Kit Plus

His-tag, Strep-tag, Signal peptide

2

RTS Wheat Germ LinTempGen Set, His6-tag

His-tag

3

RTS Linear Template Fab Kit

His-tag, Signal peptide 

4

RTS 100 E. coli LinTempGen Set, His-tag

His-tag

 
No. 


T7 vectors for cloning genes or linear templates

 
Protein tag 

5

RTS pIX4.0 Insect Vector

Tag from cloned gene

6

RTS pIX3.0 Vector

Tag from linear template

7

RTS pIVEX Wheat Germ His6-tag Vector Set

His-tag

8

RTS pIVEX E. coli His-tag, 2nd Gen. Vector Set

His-tag