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Cell-free Kits - Overview

 

Cell-free Protein Synthesis Kits

Cell-free synthesis kit

Reaction
yield

Reaction
volume

Reaction
time (hrs)

Expression
template

Expression
mode

Protein classes expressed

RTS 100 E. coli HY Kit

20 μg

50 μl

1–4

Linear: 1, 4, 5
Vector: 7, 9

Batch

Standard soluble
Membrane*

RTS 500 ProteoMaster
E. coli  HY Kit

6 mg

1 ml

6–24

Vector: 7, 9

CECF

Standard soluble
Membrane*

RTS 9000 E. coli HY Kit 

50 mg

10 ml

6–24

Vector: 7, 9

CECF

Standard soluble
Membrane*

EasyXpress E. coli Kit

20 μg
400 μg

50 μl
1 ml

1

Linear: 3
Vector: 10

Batch

Standard soluble
Membrane*

RTS 100 E. coli Fab Kit

30 μg

400 μl

6–24

Linear: 1, 4
Vector: 7, 9

Batch

Standard soluble, Disulfide-
bonded, Fab antibodies

RTS 100 E. coli Disulfide Kit

80 μg

50 μl

6–24

Vector: 7, 9

CECF

Standard soluble, Disulfide-
bonded, (Fab antibodies)

RTS 500 E. coli Disulfide Kit 

2.5 mg

1 ml

6–24

Vector: 7, 9

CECF

Standard soluble, Disulfide-
bonded, (Fab antibodies)

RTS 100 Wheat Germ Kit

5 μg

50 μl

3

Linear: 2
Vector: 8

Batch

Complex and large, Standard
Membrane*

RTS 100 Wheat Germ CECF Kit

50 μg

50 μl

6–24

Linear: 2
Vector: 8

CECF

Complex and large, Standard
Membrane*

RTS 500 Wheat Germ CECF Kit 

1 mg

1 ml

6–24

Linear: 2
Vector: 8

CECF

Complex and large, Standard
Membrane*

RTS 100 Insect Membrane Kit

2 μg

50 μl

4

Linear: 1, 3, 4, 5
Vector: 6, 7, 9

Batch

Complex and large, Standard
Membrane

RTS 100 Site-Specific Label Kit

150 μg

50 μl

1–24

Linear: 1, 4, 5
Vector: 7, 9

CECF

Standard soluble,
Disulfide-bonded, Membrane*

    RTS: Rapid (cell-free) translation system   |   CECF: Continuous exchange cell-free expression   |   Batch: Expression in batch format   |   * using detergents

Linear Templates and Vectors for Cell-Free Protein Synthesis

No.

PCR generated linear template

Protein tag

1

RTS Linear Template Kit Plus

His-tag, Strep-tag, Signal peptide

2

RTS Wheat Germ LinTempGen Set, His6-tag

His-tag

3

EasyXpress Linear Template Kit Plus
His-tag, Strep-tag, Signal peptide

4

RTS Linear Template Fab Kit

His-tag, Signal peptide 

5

RTS 100 E. coli LinTempGen Set, His-tag

His-tag

 
No. 


T7 vectors for cloning genes or linear templates

 
Protein tag 

6

RTS pIX4.0 Insect Vector

Tag from cloned gene

7

RTS pIX3.0 Vector

Tag from linear template

8

RTS pIVEX Wheat Germ His6-tag Vector Set

His-tag

9

RTS pIVEX E. coli His-tag, 2nd Gen. Vector Set

His-tag

10

EasyXpress pIX3.0 Vector

Tag from linear template