LOCUS       pIVEX1.3-CAT                4207 bp ds-DNA     circular SYN
DEFINITION  Control Vector CAT
ACCESSION   .
VERSION     .
KEYWORDS    pIVEX1.3-CAT; RTS; cell-free protein synthesis
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   biotechrabbit GmbH
            Volmerstr. 9, 12489 Berlin, Germany
COMMENT     CAT (chloramphenicol acetyltransferase) expression vector with 
            C-terminal 6xHis-tag for the control reaction.
            Ordering information: 
            BR1400201  RTS 500 ProteoMaster E. coli HY Kit
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="synthetic DNA construct"
                     /mol_type="circular plasmid"
     primer_bind     143..162
                     /label=T7-fwd
                     /note="5' sequencing primer"
     promoter        143..161
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             251..943
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase with C-terminal
                     6xHis-tag"
                     /label=CAT-6xHis
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGAPGG
                     GSHHHHHH"
     primer_bind     complement(1034..1052)
                     /label=T7-rev
                     /note="3' sequencing primer"
     terminator      1048..1095
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1913..2501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2672..3532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3533..3637)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
       61 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
      121 cgagatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
      181 tctagaaata attttgttta actttaagag gtatatatta atgtatcgat taaataagga
      241 ggaataaacc atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      301 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      361 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      421 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaactcc gtatggcaat
      481 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      541 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      601 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      661 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
      721 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcacgatgg gcaaatatta
      781 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      841 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      901 cggggcgccc ggggggggtt ctcatcatca tcatcatcat taataaaagg gcgaattcca
      961 gcacactggc ggccgttact agtggatccg gctgctaaca aagcccgaaa ggaagctgag
     1021 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     1081 ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt
     1141 cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg
     1201 gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata
     1261 tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa
     1321 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc
     1381 gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt
     1441 taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt
     1501 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1561 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1621 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1681 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1741 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1801 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1861 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1921 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1981 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2041 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2101 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2161 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2221 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2281 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2341 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2401 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2461 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2521 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2581 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2641 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2701 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2761 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2821 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2881 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2941 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3001 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3061 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3121 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3181 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3241 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3301 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3361 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3421 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3481 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3541 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3601 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3661 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3721 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     3781 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     3841 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     3901 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3961 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4021 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     4081 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat
     4141 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     4201 aacaagc
//
