LOCUS       pIVEX2.3-GFP                4235 bp ds-DNA     circular SYN
DEFINITION  Control Vector GFP
ACCESSION   .
VERSION     .
KEYWORDS    pIVEX2.3-GFP; RTS; cell-free protein synthesis
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   biotechrabbit GmbH
            Volmerstr. 9, 12489 Berlin, Germany
COMMENT     GFP (green-fluorescent protein) expression vector with C-terminal 
            6xHis-tag for the control reaction.
            Ordering information: 
            BR1400101  RTS 100 E. coli HY Kit
            BR1400102  RTS 100 E. coli HY Kit
            BR1400301  RTS 9000 E. coli HY Kit
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="synthetic DNA construct"
                     /mol_type="circular plasmid"
     primer_bind     172..191
                     /label=T7-fwd
                     /note="5' sequencing primer"
     promoter        172..190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             222..244
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             251..1000
                     /codon_start=1
                     /gene="GFP"
                     /product="Aequoria victoria green-fluorescent protein with
                     C-terminal 6xHis-tag"
                     /label=GFP-6xHis
                     /translation="MTKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYQPGGGSHHHHHH"
     primer_bind     complement(1091..1109)
                     /label=T7-rev
                     /note="3' sequencing primer"
     terminator      1105..1152
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1970..2558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2729..3589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3590..3694)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
       61 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
      121 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
      181 tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag
      241 gagatatacc atgactaaag gtgaagaact tttcactgga gttgtcccaa ttcttgttga
      301 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      361 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      421 gccaacactt gtcactactt tctcttatgg tgttcaatgc ttttcaagat acccggatca
      481 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      541 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      601 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      661 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
      721 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
      781 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      841 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
      901 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactgta
      961 ccaacccggg gggggttctc atcatcatca tcatcattaa taaaagggcg aattccagca
     1021 cactggcggc cgttactagt ggatccggct gctaacaaag cccgaaagga agctgagttg
     1081 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     1141 aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc
     1201 catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc
     1261 aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag
     1321 cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag
     1381 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
     1441 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa
     1501 ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattcgt
     1561 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1621 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1681 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     1741 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1801 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1861 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1921 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1981 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2041 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2101 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2161 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2221 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2281 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2341 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2401 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2461 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2521 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2581 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2641 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2701 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2761 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2821 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2881 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2941 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3001 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3061 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3121 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3181 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3241 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3301 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3361 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3421 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3481 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3541 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3601 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3661 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3721 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     3781 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     3841 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3901 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     3961 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     4021 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     4081 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     4141 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgca
     4201 aggagatggc gcccaacagt cccccggcca cgggg
//
