LOCUS       pIX3.0                2697 bp ds-DNA     circular SYN
DEFINITION  Cloning vector pIX3.0
ACCESSION   .
VERSION     .
KEYWORDS    pIX3.0; RTS; cell-free protein synthesis
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   biotechrabbit GmbH
            Volmerstr. 9, 12489 Berlin, Germany
COMMENT     pIX3.0 vector for easy cloning of PCR products generated using the
            RTS Linear Template Kit Plus.
            Ordering information: 
            BR1402701  RTS pIX3.0 Vector
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="synthetic DNA construct"
                     /mol_type="circular plasmid"
     primer_bind     224..241
                     /label=M13-fwd
                     /note="5' sequencing primer"
     misc_feature    251..305
                     /label=MCS
                     /note="multiple cloning site for the insertion of the 
                     target gene"
     primer_bind     complement(321..339)
                     /label=M13-rev
                     /note="3' sequencing primer"
     rep_origin      complement(723..1311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1482..2342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2343..2447)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
       61 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
      121 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
      181 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
      241 gccaagcttt ctcgagcggc cgctagcatg ccatggatat caggcctgga tccgaattca
      301 ccggtgcaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      361 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      421 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      481 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      541 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      601 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      661 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      901 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      961 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1021 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1081 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1141 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1201 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1261 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1321 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1381 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1441 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1501 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1561 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1621 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1681 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1741 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1801 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1861 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1921 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1981 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2041 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2101 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2161 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2221 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2281 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2341 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2401 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2461 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2521 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2581 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2641 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggca
//
