LOCUS       pIX4.0-Luc                4804 bp ds-DNA     circular SYN
DEFINITION  Control Vector Luc
ACCESSION   .
VERSION     .
KEYWORDS    pIX4.0-Luc; RTS; cell-free protein synthesis
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   biotechrabbit GmbH
            Volmerstr. 9, 12489 Berlin, Germany
COMMENT     Luc (firefly luciferase) expression vector with C-terminal 6xHis-tag 
            for the control reaction.
            Ordering information: 
            BR1401501  RTS 100 Insect Membrane Kit
            BR1501502  RTS 100 Insect Membrane Kit
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="synthetic DNA construct"
                     /mol_type="circular plasmid"
     primer_bind     172..191
                     /label=T7-fwd
                     /note="5' sequencing primer"
     promoter        172..190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    198..248
                     /label=5'-UTR
                     /note="5'-polyhedrin-UTR"
     CDS             251..1927
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase (Photinus pyralis) with 
                     C-Terminal 6xHis-tag"
                     /label=Luc-6xHis
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAVWGHHHHHH"
     misc_feature    1970..1999
                     /label=polyA
                     /note="polyadenylation site, adds 30 A to the mRNA"
     primer_bind     complement(2050..2068)
                     /label=T7-rev
                     /note="3' sequencing primer"
     terminator      2064..2111
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    2114..2136
                     /label=Linearization site
                     /note="Linearization site for production of run-off 
                     transcripts.
                     blunt ends: DraI, EcoRV, SmaI, StuI, SwaI
                     5'-overhang: EcoRI, XmaI"
     rep_origin      complement(2553..3141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3312..4172)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4173..4277)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
       61 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
      121 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc ctaatacgac
      181 tcactatagg gtctagataa gtattttact gttttcgtaa cagttttgta ataaaaaaac
      241 ctataaatcc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct
      301 ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
      361 tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt
      421 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
      481 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
      541 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag
      601 tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt
      661 gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga
      721 ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa
      781 tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa
      841 ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt
      901 gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat
      961 tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat
     1021 atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct
     1081 tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa
     1141 aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc
     1201 tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag
     1261 gcaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga
     1321 taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga
     1381 taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat
     1441 tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg
     1501 gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg
     1561 cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat
     1621 cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc
     1681 cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga
     1741 gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt
     1801 gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga
     1861 gatcctcata aaggccaaga agggcggaaa gatcgccgtg tggggtcacc accatcacca
     1921 tcattaataa ctaactaacc aagatctaaa taataagtaa ttaactagta aaaaaaaaaa
     1981 aaaaaaaaaa aaaaaaaaag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     2041 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     2101 ggggtttttt gatatcccgg gatttaaata ggcctgaatt cgtaatcatg gtcatagctg
     2161 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     2221 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     2281 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     2341 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2401 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2461 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2521 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2581 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2641 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2701 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2761 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2821 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2881 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2941 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     3001 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3061 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3121 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3181 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3241 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3301 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3361 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3421 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3481 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3541 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3601 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3661 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3721 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3781 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3841 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3901 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3961 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4021 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4081 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4141 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4201 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4261 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     4321 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     4381 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     4441 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     4501 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     4561 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     4621 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     4681 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     4741 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcaaggagat ggcgcccaac
     4801 agtc
//
