LOCUS       pIX4.0                3144 bp ds-DNA     circular SYN
DEFINITION  Cloning vector pIX4.0
ACCESSION   .
VERSION     .
KEYWORDS    pIX4.0; RTS; cell-free protein synthesis
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   biotechrabbit GmbH
            Volmerstr. 9, 12489 Berlin, Germany
COMMENT     pIX4.0 Insect Vector for generation of optimal expression templates
            to be used with the RTS 100 Insect Membrane Kit.
            Ordering information: 
            BR1400901  RTS pIX4.0 Insect Vector
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="synthetic DNA construct"
                     /mol_type="circular plasmid"
     primer_bind     172..191
                     /label=T7-fwd
                     /note="5' sequencing primer"
     promoter        172..190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    198..248
                     /label=5'-UTR
                     /note="5'-Polyhedrin-UTR"
     misc_feature    249..309
                     /label=MCS
                     /note="multiple cloning site for the insertion of the 
                     target gene"
     CDS             251..253
                     /codon_start=1
                     /label=Start
                     /note="start of translation"
                     /translation="M"
     misc_feature    310..339
                     /label=polyA
                     /note="polyadenylation site, adds 30 A to the mRNA"
     primer_bind     complement(390..408)
                     /label=T7-rev
                     /note="3' sequencing primer"
     terminator      404..451
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    454..476
                     /label=Linearization site
                     /note="Linearization site for production of run-off 
                     transcripts.
                     blunt ends: DraI, EcoRV, SmaI, StuI, SwaI
                     5'-overhang: EcoRI, XmaI"
     rep_origin      complement(893..1481)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1655..2512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2513..2617)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
       61 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
      121 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc ctaatacgac
      181 tcactatagg gtctagataa gtattttact gttttcgtaa cagttttgta ataaaaaaac
      241 ctataaatcc atgggagacc cctcctcgag cggccgcctg cagatctaaa taataagtaa
      301 ttaactagta aaaaaaaaaa aaaaaaaaaa aaaaaaaaag gatccggctg ctaacaaagc
      361 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      421 ggcctctaaa cgggtcttga ggggtttttt gatatcccgg gatttaaata ggcctgaatt
      481 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      541 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      601 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      661 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      721 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      781 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      841 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      901 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      961 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1021 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1081 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1141 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1201 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1261 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1321 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1381 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1441 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1501 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1561 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1621 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1681 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1741 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1801 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1861 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1921 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1981 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2041 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2101 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2161 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2221 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2281 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2341 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2401 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2461 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2521 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2581 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2641 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2701 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     2761 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     2821 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     2881 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2941 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     3001 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     3061 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat
     3121 gcaaggagat ggcgcccaac agtc
//
