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Reverse Transcription & RT-PCR

RevertUP Reverse Transcriptase, which is a proprietary modification of the MMuLV reverse transcriptase, provides most efficient cDNA synthesis without RNase H activity, allowing successful synthesis of cDNAs of greater than 14 kb in length.
One-step RT-PCR kits provide optimized reagents, including Hot Start Taq DNA Polymerase, for performing both reverse-transcription and amplification reactions in one tube.
The Moloney-Murine Leukemia Virus (MMuLV) reverse transcriptase is a classic RNA-dependent DNA polymerase. The exceptional purity of the biotechrabbit MMuLV enzyme ensures excellent results in demanding applications. A special RNase Inhibitor prevents RNA templates from degradation by pervasive RNases.

High performance — exceptional purity for demanding applications
Convenient — excellent efficiency at high temperatures
Reliable — RNase Inhibitor for protecting your RNA

 

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  1. RevertUP™ II Reverse Transcriptase, 200 U/µl

    RevertUP™ II Reverse Transcriptase, 200 U/µl

    Features
    • Improved performance for synthesis of long cDNAs (≥ 19 kb)
    • Excellent stability at high temperatures up to 60°C
    • High sensitivity for cDNA synthesis from few copies of template
    Applications
    • First-strand cDNA synthesis
    • Generation of labeled cDNA
    • RNA analysis by primer extension
    • cDNA library construction
    • RT-PCR
    Learn More…

    Package Sizes
    Starting at:
    €85.00

    Does not include applicable tax, shipping & handling, or other charges.

  2. MMuLV Reverse Transcriptase, 200 U/µl

    MMuLV Reverse Transcriptase, 200 U/µl

    Features
    • Pure reverse transcriptase for cDNA synthesis
    • High yields of first-strand cDNA
    • High value for a fair price
    Applications
    • First-strand cDNA synthesis
    • Generation of labeled cDNA
    • RNA analysis by primer extension
    Learn More…

    Package Sizes
    Starting at:
    €55.00

    Does not include applicable tax, shipping & handling, or other charges.

  3. One Step RT PCR Kit

    One Step RT PCR Kit

    Features
    • Efficient thermostable Reverse Transcriptase and RNase Inhibitor providing high cDNA yields
    • Unique Hot Start Taq DNA Polymerase in a mix with high-quality dNTPs
    • PCR enhancers allowing sensitive low background amplification
    Applications
    • One-step RT-PCR
    • Virus detection
    • Amplification of GC-rich and complex templates
    Learn More…

    Package Sizes
    Starting at:
    €119.00

    Does not include applicable tax, shipping & handling, or other charges.

  4. cDNA Synthesis Kit

    cDNA Synthesis Kit

    Features
    • Highly efficient synthesis of long cDNAs (≥ 19 kb)
    • Excellent yields at temperatures up to 55°C
    • High sensitivity reverse transcription from low abundance template
    • Superior performance in demanding applications, including templates with a high degree of secondary structure
    Applications
    • First-strand cDNA synthesis for RT-PCR and qPCR
    • Gene expression profiling
    • RNA labeling and primer extension
    • cDNA library construction
    Learn More…

    Package Sizes
    Starting at:
    €54.00

    Does not include applicable tax, shipping & handling, or other charges.

  5. RNase Inhibitor, 40 U/µl

    RNase Inhibitor, 40 U/µl

    Features
    • Exceptionally pure proprietary Ribonuclease Inhibitor for demanding RNA applications
    • Active under variety of reaction conditions used for work with RNA
    • Prevention of RNA from degradation by a wide range of RNases
    Applications
    • In vitro transcription/translation
    • cDNA synthesis
    • RNA purification and storage
    Learn More…

    Package Sizes
    Starting at:
    €53.00

    Does not include applicable tax, shipping & handling, or other charges.

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