biotechrabbit™ GenUP Exo SAP Kit is designed for quickly and easily cleaning up PCR amplification reactions, enabling their direct use in sequencing or genotyping.
The kit provides a combination of Shrimp Alkaline Phosphatase (SAP) and Exonuclease 1 (ExoI) for removing nucleotides and primers. Double-stranded DNA is not affected, ensuring 100% recovery of PCR products.
Both enzymes are active in PCR buffers and completely inactivated by heating at 80°C: no further processing is required. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, and the 3' → 5' exonuclease activity of Exonuclease 1 degrades single-stranded primers.
Component | Composition |
ExoI-SAP | Exonuclease I and SAP in storage buffer containing 50% glycerol (v/v). |
STORAGE | −20°C (until expiry date – see product label) |
Quality Control
Shrimp Alkaline Phosphatase
Specificity
SAP Removes 5’-phosphates from DNA, RNA, dNTPs and proteins.
Purity
Purified to apparent homogeneity. SAP is highly pure and tested free of contaminating nucleases.
Exonuclease 1
Specificity
ExoI is a 3’-5’ exonuclease, specific for single stranded DNA.
Purity
Purified to apparent homogeneity. ExoI is highly pure and tested free of contaminating nucleases.
STEPS |
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For detailed protocol, please, refer to the product manual (Related Documents).
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