biotechrabbit™ GenUP Plant RNA Kit has been developed for quick and easy purification of total RNA from plant materials. After initial homogenization and lysis, genomic DNA is bound to a Mini Filter DNA, which can be discarded. RNA is selectively bound to a Mini Filter RNA, washed with two different buffers and eluted. The purified RNA is ready for use in any demanding molecular biology application, including RT-PCR.
Two lysis buffers, Buffer LYSIS LR and Buffer LYSIS LT, are provided to maximize yield. Most plant material can be processed with Buffer LYSIS LR. In the cases that yield using Buffer LYSIS LR is low, use Buffer LYSIS LT.
STARTING MATERIAL | Plant material (up to 100 mg) |
EXTRACTION TIME | 30 min after homogenization |
BINDING CAPACITY | Approximately 100 µg RNA |
TYPICAL YIELD | Variable depending on the starting material; approximately 70 µg RNA |
AVERAGE PURITY | A260/A280 1.7–2.0 |
STEPS |
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For detailed protocol, please, refer to the product manual (Related Documents).
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