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StranDisplace™ II Thermostable DNA Polymerase, 8 U/µl

Features

  • Exceptionally pure thermostable DNA polymerase
  • Strong strand-displacement activity
  • High salt and non-ionic detergent tolerance
  • Deficient in 5'→3' and 3'→5' exonuclease activity

Applications

  • Isothermal nucleic acid amplification/detection
  • Very rapid and sensitive detection via LAMP assays
  • Sequencing

Purchase StranDisplace™ II Thermostable DNA Polymerase, 8 U/µl

Availability: In stock Bulk requests please inquire at oem@biotechrabbit.com

Catalog # Size Package Content Price* Qty
BR1101301 8000 U | 1000 rxn 1 ml StranDisplace II Thermostable DNA Polymerase
2 × 1.5 ml 10× SD II Reaction Buffer
€290.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit™ StranDisplace II Thermostable DNA Polymerase is an exceptionally pure enzyme for isothermal nucleic acid amplification/detection applications in which strong strand-displacement activity at elevated temperatures is required. The polymerase is especially well suited for very rapid and sensitive detection via LAMP assays.

StranDisplace II Thermostable DNA Polymerase is a thermophilic DNA polymerase with strong strand-displacement activity and deficiency in both, 3'→ 5' and 5'→ 3' nuclease activities. The enzyme tolerates elevated salt concentrations up to 125 mM KCl, and non-ionic detergents detergents up to 5%.

The polymerase is active up to 70°C and can be used for the same applications as Bst DNA Polymerase Large Fragment and Bsm DNA Polymerase.

 

Component

Composition

StranDisplace II Thermostable DNA Polymerase

StranDisplace II Thermostable DNA Polymerase, 8 U/µl, in storage buffer containing 50% (v/v) glycerol.

10× SD II Reaction Buffer

Optimized 10× SD II reaction buffer.

 

STORAGE

-20°C (until expiry date – see product label)

 

Unit Definition

One unit is defined as the amount of polymerase required to convert 10 nmol of dNTP into acid insoluble material in 30 minutes at 30°C.

Quality Control
Protein Purity

Protein purity is analyzed by SDS polyacrylamide gel electrophoresis.

Exonuclease Activity

Linearized DNA is incubated with the enzyme in a 50 µl reaction mixture for 4 h at 37°C. No degradation of DNA was observed.

Endonuclease/Nick Activity

Supercoiled plasmid DNA is incubated with the enzyme in a 50 µl reaction mixture for 4 h at 37°C. No conversion of covalently closed circular DNA to nicked DNA was detected.

Contamination with E. coli DNA

A sample of denatured enzyme is analyzed with specific primers targeting the 16S rRNA gene in qPCR for the presence of contaminating E. coli DNA. No E. coli DNA was detectable.

 

Application recommendation
  • Refer to the public articles for recommendations and protocols.
  • Recommended use is 8 units per reaction.
  • Recommended temperature for isothermal amplification is 60-65⁰C.
  • The enzyme is heat inactivated by incubation at 80°C for 10 minutes.
  • Use of this enzyme in certain applications may be covered by patents and may require a license.

 


References
Loop-mediated isothermal amplification (LAMP)
  • Tsugunori Notomi, et al., 2000, Nucleic Acids Res., v. 28, No. 12, e63.
  • Masaki Imai, et al., 2007, Journal of Virological Methods, 141, 173-180, 2007.
Sequencing
  • Mead, D.A. et al., 1991, Biotechniques, 11, 76-87.

Loop-mediated isothermal amplification (LAMP)

  • Tsugunori Notomi, et al., 2000, Nucleic Acids Res., v. 28, No. 12, e63.
  • Masaki Imai, et al., 2007, Journal of Virological Methods, 141, 173-180, 2007.

Sequencing

  • Mead, D.A. et al., 1991, Biotechniques, 11, 76-87.
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