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RTS 9000 E. coli HY Kit
Features
- Unique CECF technology to ensure high yields
- Expression of toxic proteins that cannot be produced in vivo
- Incorporation of labeled methionine into proteins
Applications
- Large-scale, cell-free expression of up to 50 mg protein
Package Sizes
Starting at: €4,990.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 500 E. coli Disulfide Kit
Features
- Optimized reaction conditions for the expression of functional disulfide-bonded proteins
- No need for refolding aggregated proteins
- CECF technology for yields of up to 2.5 mg
Applications
- Expression of active proteins containing multiple disulfide bonds
Package Sizes
Starting at: €2,560.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 500 Wheat Germ CECF Kit
Features
- Successful expression of eukaryotic proteins , generally without sequence optimization
- Synthesis of up to 1 mg functional eukaryotic protein
- Maximized production of active heat-sensitive proteins, due to an optimum reaction temperature of 24°C
Applications
- Up to milligram-scale protein expression of functional eukaryotic proteins
- Expression of multiple proteins in parallel
Package Sizes
Starting at: €2,560.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 500 ProteoMaster E. coli HY Kit
Features
- Unique CECF technology to ensure high yields
- Enable expression of toxic proteins that cannot be produced in vivo
- Incorporation of labeled methionine into synthesized proteins
Applications
- Optimized medium-scale, cell-free expression of up to 6 mg protein
- Protein synthesis for NMR spectroscopy or X-ray crystallography
Package Sizes
Starting at: €2,535.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 100 Wheat Germ CECF Kit
Features
- Successful expression of eukaryotic proteins , generally without sequence optimization
- Synthesis of up to 50 µg functional eukaryotic protein
- Maximized production of active heat-sensitive proteins, due to an optimum reaction temperature of 24°C
Applications
- Up to milligram-scale protein expression of functional eukaryotic proteins
- Expression of multiple proteins in parallel
Package Sizes
Starting at: €925.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 100 E. coli Disulfide Kit
Features
- Optimized reaction conditions for the expression of functional disulfide-bonded proteins
- No need for refolding aggregated proteins
- CECF technology for yields of up to 80 µg
Applications
- Expression of active proteins containing multiple disulfide bonds
Package Sizes
Starting at: €825.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 100 Wheat Germ Kit
Features
- Efficient expression of up to 5 μg protein per reaction
- Compatible with non-optimized, pro- and eukaryotic T7 promoter-based vectors as well as linear templates
- Excellent homogeneity of large proteins (264 kDa expressed)
- No endotoxins, allows direct use in cell-based assays
- Upscaling using high yield CECF wheat germ kits
Applications
- Fast parallel synthesis for screening purposes
- Synthesis of mammalian or other proteins for functional testing without the disturbing endogenous protein background that is typical for in vivo expression
- Synthesis of proteins dependent on eukaryotic chaperones
Package Sizes
Starting at: €740.00Does not include applicable tax, shipping & handling, or other charges.
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RTS pIVEX E. coli His-tag, 2nd Gen. Vector Set
Features
- Stable circular templates to ensure high yields in preparative-scale expressions
- For construction of C- and N-terminal His6-tagged proteins
- Optimal spacing of all regulatory elements
- For use with RTS E. coli and Insect kits
Applications
- Preparative-scale expression of His6-tagged proteins
Package Sizes
Starting at: €415.00Does not include applicable tax, shipping & handling, or other charges.
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RTS pIVEX Wheat Germ His6-tag Vector Set
Features
- Stable circular templates ensure high yields in preparative-scale expression reactions
- For construction of C- and N-terminal His6-tag proteins
Applications
- Expression vector for preparative-scale expression in eukaryotic wheat germ lysates
Package Sizes
Starting at: €410.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 100 E. coli LinTempGen Set, His-tag
Features
- Contains all regulatory elements in optimal spacing
- Option for templates with N- or C-terminal His6-tag
- Primary PCR product can be cloned into pIVEX vectors
- For use with RTS 100 E. coli HY and insect kits
Applications
- Fast gene template generation without cloning
- Generate linear templates for rapid screening and optimization of His6-tagged protein expression
Package Sizes
Starting at: €400.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Linear Template Fab Kit
Features
- Introduction of an affinity tag for protein purification
- Signal peptide sequence for translocation into microsomes
- Introduction of a signal peptide sequence for translocation into microsomes
- Compatibility with E. coli HY, Fab and Insect kits
Applications
- PCR-based inear templates for protein expression in E. coli and insect disulfide kits
- Fast template generation for expression of Fab and scFv antibody fragments
Package Sizes
Starting at: €360.00Does not include applicable tax, shipping & handling, or other charges.
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Tris-NTA Biotin
Features
- A complex of three Ni-NTA groups ensures high-affinity binding of His-tags
- Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators
- Protein binding is stoichiometric
Applications
- Reversible labeling of proteins or cell surfaces
- Detection and analysis of target molecules
- Immobilization of proteins, lipids and cells on surfaces
- Purification and sample preparation of proteins
- Coupling with microscopic or spectroscopic probes
Package Sizes
Starting at: €360.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Wheat Germ LinTempGen Set, His6-tag
Features
- Optimized spacing of eukaryotic regulatory elements
- Time saving, no laborious cloning
- Flexible positioning of His6-tag at the C- or N-terminus
- Primary PCR product can be cloned into pIVEX vectors
Applications
- Rapid generation of linear expression constructs for screening applications
- Linear template generation for optimized His6-tagged protein expression
Package Sizes
Starting at: €350.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Linear Template Kit Plus
Features
- Efficient in vitro expression (E. coli and insect kits)
- Primers for affinity tags (His6-tag /Strep-tag II)
- Signal peptide sequence primers for expression translocation into microsomes (insect systems)
Applications
- Fast generation of multiple template constructs
- Accelerated screening of protein expression templates and conditions
Package Sizes
Starting at: €345.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 100 Insect Membrane Kit
Features
- Robust expression of a wide range of proteins (clotting factors, protein kinases, transcription factors)
- Yields of up to 50 μg/ml protein, sufficient for functional studies or screening procedures
- Compatibility with linear templates and plasmids
- Expression screening in small volumes (>5 μl)
Applications
- Expression of membrane proteins without the need for additives • Synthesis of pos
- Synthesis of post-translationally modified eukaryotic proteins
Package Sizes
Starting at: €235.00Does not include applicable tax, shipping & handling, or other charges.
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5X CAPITAL™ 1-Step qRT-PCR Probe Master Mix, lyophilized
Features
- Stable enzyme and mix for ambient shipment and room-temperature storage
- Best in-class performance for both single and multiplex detection
- Convenient master mix for detection of low-copy pathogen targets
- High specificity and sensitivity across a wide range of sample sources
Applications
- One step qRT-PCR from mRNA, total RNA and viral RNA targets
- For use with standard and fast qPCR platforms
- Single and multiplex qRT-PCR reactions
Package Sizes
Starting at: €225.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 100 E. coli HY Kit
Features
- Compatible with linear templates for fast screening without cloning
- Convenient reaction setup
- Fast expression of proteins in parallel
- Improved high yield biochemistry for up to 20 μg protein per reaction
Applications
- Fast parallel microgram-scale protein expression
Package Sizes
Starting at: €215.00Does not include applicable tax, shipping & handling, or other charges.
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Tris-NTA Amine
Features
- A complex of three Ni-NTA groups ensures high-affinity binding of His-tags
- Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators
- Protein binding is stoichiometric
Applications
- Reversible labeling of proteins or cell surfaces
- Detection and analysis of target molecules
- Immobilization of proteins, lipids and cells on surfaces
- Purification and sample preparation of proteins
- Coupling with microscopic or spectroscopic probes
Package Sizes
Starting at: €215.00Does not include applicable tax, shipping & handling, or other charges.
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2X Hot-Start PCR Master Mix, lyophilized
Features
- Room-temperature stable enzymes and mixes
- Exceptionally pure Taq DNA Polymerase
- Hot-start for high PCR specificity and sensitivity
- Optimized Master Mix for fast setup
Applications
- Ambient shipment and room-temperature storage
- Routine and demanding PCR applications
- PCR amplification up to 3 kb
- TA cloning
Package Sizes
Starting at: €149.00Does not include applicable tax, shipping & handling, or other charges.
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2X YourTaq™ Hot-Start PCR Master Mix, lyophilized
Features
- Stable enzyme and mix for ambient shipment and room-temperature storage
- Exceptionally pure YourTaq Hot Start DNA Polymerase
- Optimized Master Mix for increased yield of amplification
- Resistant to PCR inhibitor carry-over
- Excellent PCR specificity and sensitivity for a broad range of amplicons
Applications
- Routine and demanding PCR amplification up to 3 kb
- Suitable for amplification of low target copy number
- TA cloning
Package Sizes
Starting at: €149.00Does not include applicable tax, shipping & handling, or other charges.