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GenUP™ Mycobacteria gDNA Kit

Features

  • Fast and simple procedure
  • gDNA from sputum samples, bronchoalveolar lavage and tissue biopsies
  • High yields of pure DNA for demanding applications

Applications

  • Isolation of genomic DNA from mycobacteria

Purchase GenUP™ Mycobacteria gDNA Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0702201 10 preps Solutions, Mini Filters and Vials
€61.00
BR0702202 50 preps Solutions, Mini Filters and Vials
€202.00
BR0702203 250 preps Solutions, Mini Filters and Vials
€825.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit™ GenUP Mycobacteria gDNA Kit has been specially developed for quick and easy purification of mycobacterial genomic DNA from various sources including sputum samples, bronchoalveolar lavage and tissue biopsies.

In a first purification step the sample is pre-treated for enrichment of mycobacteria. A following combined lysozyme and proteolytic lysis steps allow efficient cell disruption. The DNA is bound to a high-capacity filter, washed and then eluted in a separate tube. The purified DNA is ready to be used in all demanding molecular biology applications, including PCR, enzymatic digestions, cloning and other.


STARTING MATERIAL

0.2–5 ml sputum sample, up to 1 ml bronchoalveolar lavage or 1–10 mg tissue biopsies (e.g., lymph nodes)

EXTRACTION TIME

Approximately 15 min after lysis step

BINDING CAPACITY

> 50 µg DNA

TYPICAL YIELD

Variable; yield depends on type of mycobacteria and amount of starting material


STEPS

  • Pre-treat samples with Buffer DECONTAMINATION. Centrifuge.
  • Resuspend the cell pellet in TE buffer.
  • Add lysozyme and incubate.
  • Add Buffer LYSIS LB and Proteinase K, mix and incubate.
  • Centrifuge to pellet unlysed material
  • Transfer the lysate to a new tube and add Buffer BINDING BG.
  • Apply the mixture to a Mini Filter and centrifuge.
  • Wash with Buffer WASH A and Buffer WASH C and centrifuge.
  • Centrifuge again to remove residual ethanol.
  • Elute DNA in Buffer ELUTION.
  • Purified DNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

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