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GenUP™ Virus RNA Kit

Features

  • Universal kit for isolating viral RNA from various starting materials
  • Fast and simple procedure, flexible elution volumes
  • High yields of pure RNA
  • No DNase treatment, no toxic β-mercaptoethanol

Applications

  • Virus RNA isolation from plasma, serum, urine, other body fluids, cell cultures, tissues, and buccal swabs

Purchase GenUP™ Virus RNA Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0701001 10 preps Solutions, Mini Filters and Vials
€58.00
BR0701002 50 preps Solutions, Mini Filters and Vials
€190.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit™ GenUP Virus RNA Kit has been specially developed for quick and easy isolation of viral RNA. Viral single-stranded RNA can be isolated from eukaryotic samples including plasma, serum, urine, and other body fluids as well as cell cultures, tissues, and buccal swabs.

The unique binding membrane of our high-capacity Mini Filters guaranties high yields. A high concentration of purified RNA can be achieved with flexible elution volumes. The kit includes carrier RNA.

After few initial procedures, the viral RNA is bound to a Mini Filter, washed and then eluted in a separate tube. The purified RNA is ready to be used in all demanding molecular biology applications, including cDNA synthesis, northern blot analysis, qPCR and RT-PCR.


STARTING MATERIAL

Eukaryotic cells (up to 5 × 106)
Serum, plasma, cell-free body fluids, cell culture supernatants (150 µl)
Tissue samples, biopsies (up to 20 mg)
Paraffin-embedded tissues, buccal swabs

EXTRACTION TIME

Approximately 25 min

TYPICAL YIELD

Yield is highly dependent on sample type


STEPS

  • Homogenize and lyse the sample material.
  • Centrifuge to pellet debris.
  • Add Buffer BINDING BR and transfer to a Mini Filter (violet).
  • Centrifuge.
  • Add Buffer WASH A and centrifuge.
  • Add Buffer WASH B and centrifuge.
  • Centrifuge once more to remove residual ethanol.
  • Add Water, RNase-free (for ELUTION), incubate and centrifuge.
  • RNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

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