LOCUS       pIVEX2.3-UK                4353 bp ds-DNA     circular SYN
DEFINITION  Control Vector UK
ACCESSION   .
VERSION     .
KEYWORDS    pIVEX2.3-UK; RTS; cell-free protein synthesis
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   biotechrabbit GmbH
            Volmerstr. 9, 12489 Berlin, Germany
COMMENT     UK (urokinase) expression vector with C-terminal 6xHis-tag for 
            the control reaction.
            Ordering information: 
            BR1400401  RTS 100 E. coli Disulfide Kit
            BR1400501  RTS 500 E. coli Disulfide Kit
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="synthetic DNA construct"
                     /mol_type="circular plasmid"
     primer_bind     172..191
                     /label=T7-fwd
                     /note="5' sequencing primer"
     promoter        172..190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             222..244
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             252..1118
                     /codon_start=1
                     /gene="PLAU"
                     /gene_synonym="u-PA; uPA"
                     /product="urokinase (Mus musculus) with C-terminal 
                     6xHis-tag"
                     /label=UK-6xHis
                     /note="Specifically cleaves the zymogen plasminogen to form
                     the active enzyme plasmin."
                     /note="255..1070:  Urokinase-type plasminogen activator 
                     chain B
                     1098-1118: 6x-His affinity tag"
                     /protein_id="P06869"
                     /translation="MVDQQGFQCGQKALRPRFKIVGGEFTEVENQPWFAAIYQKNKGGS
                     PPSFKCGGSLISPCWVASAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILH
                     EYYREDSLAYHNDIALLKIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKE
                     SESDYLYPKNLKMSVVKLVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPL
                     ICNIEGRPTLSGIVSWGRGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAFLERAPGGG
                     SHHHHHH"
     primer_bind     complement(1209..1227)
                     /label=T7-rev
                     /note="3' sequencing primer"
     terminator      1223..1270
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(2088..2676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2847..3707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3708..3812)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
       61 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
      121 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
      181 tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag
      241 gagatataca tatggtagac caacaaggct tccagtgtgg ccagaaggct ctaaggcccc
      301 gctttaagat tgttggggga gaattcactg aggtggagaa ccagccctgg ttcgcagcca
      361 tctaccagaa gaacaaggga ggaagtcctc cctcctttaa atgtggtggg agtctcatca
      421 gtccttgctg ggtggccagt gccgcacact gcttcattca actcccaaag aaggaaaact
      481 acgttgtcta cctgggtcag tcgaaggaga gctcctataa tcctggagag atgaagtttg
      541 aggtggagca gctcatcttg cacgaatact acagggaaga cagcctggcc taccataatg
      601 atattgcctt gctgaagata cgtaccagca cgggccaatg tgcacagcca tccaggtcca
      661 tacagaccat ctgcctgccc ccaaggttta ctgatgctcc gtttggttca gactgtgaga
      721 tcactggctt tggaaaagag tctgaaagtg actatctcta tccaaagaac ctgaaaatgt
      781 ccgtcgtaaa gcttgtttct catgaacagt gtatgcagcc ccactactat ggctctgaaa
      841 ttaattataa aatgctgtgt gctgcggacc cagagtggaa aacagattcc tgcaagggcg
      901 attctggagg accgcttatc tgtaacatcg aaggccgccc aactctgagt gggattgtga
      961 gctggggccg aggatgtgca gagaaaaaca agcccggtgt ctacacgagg gtctcacact
     1021 tcctggactg gattcaatcc cacattggag aagagaaagg tctggccttc ctcgagcgag
     1081 ctcccggggg gggttctcat catcatcatc atcattaata aaagggcgaa ttccagcaca
     1141 ctggcggccg ttactagtgg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     1201 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     1261 gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca
     1321 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa
     1381 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg
     1441 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc
     1501 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
     1561 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
     1621 gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcgtaa
     1681 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1741 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1801 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1861 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1921 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1981 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2041 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2101 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2161 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2221 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2281 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2341 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2401 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2461 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2521 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2581 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2641 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2701 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2761 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2821 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2881 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2941 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3001 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3061 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3121 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3181 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3241 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3301 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3361 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3421 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3481 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3541 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3601 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3661 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3721 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3781 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3841 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3901 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     3961 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4021 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     4081 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     4141 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     4201 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     4261 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcatgcaag
     4321 gagatggcgc ccaacagtcc cccggccacg ggg
//
