biotechrabbit™ GenUP Mycobacteria gDNA Kit has been specially developed for quick and easy purification of mycobacterial genomic DNA from various sources including sputum samples, bronchoalveolar lavage and tissue biopsies.
In a first purification step the sample is pre-treated for enrichment of mycobacteria. A following combined lysozyme and proteolytic lysis steps allow efficient cell disruption. The DNA is bound to a high-capacity filter, washed and then eluted in a separate tube. The purified DNA is ready to be used in all demanding molecular biology applications, including PCR, enzymatic digestions, cloning and other.
STARTING MATERIAL | 0.2–5 ml sputum sample, up to 1 ml bronchoalveolar lavage or 1–10 mg tissue biopsies (e.g., lymph nodes) |
EXTRACTION TIME | Approximately 15 min after lysis step |
BINDING CAPACITY | > 50 µg DNA |
TYPICAL YIELD | Variable; yield depends on type of mycobacteria and amount of starting material |
STEPS |
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For detailed protocol, please, refer to the product manual (Related Documents).
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