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GenUP™ Plant DNA Kit

Features

  • Simple and efficient procedure for plant DNA isolation
  • Special lysis protocols for different plant materials
  • High yields of inhibitor-free DNA

Applications

  • Universal kit for isolating genomic DNA from various plant materials

Purchase GenUP™ Plant DNA Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0700801 10 preps Solutions, Mini Filters and Vials
€51.00
BR0700802 50 preps Solutions, Mini Filters and Vials
€169.00
BR0700803 250 preps Solutions, Mini Filters and Vials
€691.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit™ GenUP Plant DNA Kit has been specially developed for quick and easy purification of genomic DNA from a wide variety of plant materials, including fresh, frozen or dried samples from leaves, roots, stems and flowers.

The kit includes an advanced prefiltration step to remove unlysed tissue. Subsequently, DNA is bound to a Mini Filter and is subsequently washed and eluted in a separate tube. The purified DNA is ready for use in any demanding molecular biology application, including PCR, enzymatic digestions and cloning.

This kit provides three buffers for optimized processing with different plant materials. To determine optimal lysis conditions, side-by-side preparation using the three provided protocols are prepared.


STARTING MATERIAL

Fresh, frozen or dried plant tissue (maximum 100 mg dry weight
or 180 mg wet weight)

EXTRACTION TIME

Approximately 40 min

BINDING CAPACITY

> 50 µg DNA

TYPICAL YIELD

Variable depending on the starting material; approximately 5–25 µg DNA

AVERAGE PURITY

A260/A280 1.7–2.0


STEPS

  • Homogenize plant material.
  • Mortar and pestle in presence of liquid nitrogen.
  • Homogenizer, e.g., rotor-stator or bead mill.
  • Lysis dependent on Buffer LYSIS:
    • add Buffer LYSIS LC, incubate and proceed with the next step.
    • add Buffer LYSIS LS or Buffer LYSIS LD, incubate, add Buffer PRECIPITATION and centrifuge to remove unlysed material.
  • Filter lysate using a Pre Filter (lavender).
  • Bind DNA to the Mini Filter (green) with Buffer BINDING BD.
  • Centrifuge to remove unbound material.
  • Wash the DNA twice with Buffer WASH C.
  • Centrifuge twice to remove the wash buffer.
  • Elute DNA with Buffer ELUTION and centrifuge.
  • Purified DNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

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