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Hot-Start PCR

Hot-start enzymes are completely inactive during room-temperature setup and become active only after heating, reducing random primer annealing and unspecific amplification that can occur with standard PCR enzymes. The fidelity of hot-start PCR is the same as for standard PCR, and targets of 3–5 kb are routinely amplified. Like standard Taq DNA polymerase, hot-start enzymes add an adenine to the 3' end of the product, allowing TA cloning.

Hot-start PCR master mixes, which contain all necessary PCR reagents, simplify PCR setup. Colored, high-density hot-start PCR buffers allow PCR products to be loaded directly onto gels after cycling. Colored storage buffers allow easy identification of mixes containing Hot Start Taq DNA Polymerase during reaction setup.

Optimized — excellent enzyme performance for enhanced applications
Specific and sensitive — hot start for better results
Convenient — component choices that make your job easier
Fast — master mixes for less pipetting during setup

 

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  1. ApStarTaq™ DNA Polymerase, 5 U/µl

    ApStarTaq™ DNA Polymerase, 5 U/µl

    Features
    • Aptamer-based hot-start functionality
    • No activation steps needed
    • High PCR specificity and sensitivity
    • Exceptionally pure Taq DNA Polymerase for sensitive PCR applications and high yields
    Applications
    • Fast PCR reactions in endpoint and real-time analysis
    • Hot-start PCR up to 3 kb
    • Amplification of low-copy-number targets
    • RT-PCR and TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €52.00

    Does not include applicable tax, shipping & handling, or other charges.

  2. Hot Start Taq DNA Polymerase, 5 U/µl

    Hot Start Taq DNA Polymerase, 5 U/µl

    Features
    • High PCR specificity and sensitivity
    • Exceptionally pure Hot Start Taq DNA Polymerase for sensitive PCR applications and high yields
    • Antibody-based Hot Start for fast polymerase activation
    Applications
    • Hot-start PCR up to 5 kb
    • Amplification of low-copy-number targets
    • RT-PCR and TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €25.00

    Does not include applicable tax, shipping & handling, or other charges.

  3. Hot Start PCR Master Mix, 2×

    Hot Start PCR Master Mix, 2×

    Features
    • Highest PCR sensitivity without prolonged reactivation
    • Optimized PCR Master Mix for minimal hands-on and fast setup
    • Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs
    Applications
    • High-specificity and high-throughput hot-start PCR up to 5 kb
    • Amplification of low-copy-number targets
    • TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €52.00

    Does not include applicable tax, shipping & handling, or other charges.

  4. Hot Start PCR Master Mix, lyophilized, 2×

    Hot Start PCR Master Mix, lyophilized, 2×

    Features
    • Room-temperature stable enzymes and mixes
    • Exceptionally pure Taq DNA Polymerase
    • Hot-start for high PCR specificity and sensitivity
    • Optimized Master Mix for fast setup
    Applications
    • Ambient shipment and room-temperature storage
    • Routine and demanding PCR applications
    • PCR amplification up to 3 kb
    • TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €38.00

    Does not include applicable tax, shipping & handling, or other charges.

  5. Green Hot Start PCR Master Mix Direct-Load, 2×

    Green Hot Start PCR Master Mix Direct-Load, 2×

    Features
    • Optimized Green Hot Start PCR Master Mix for fast setup and direct loading on the gel
    • Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs
    • High product yields and robustness in a wide application range
    Applications
    • High-throughput hot-start PCR and immediate gel analysis
    • High-specificity hot-start PCR up to 5 kb
    • Amplification of low-copy-number targets
    • TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €52.00

    Does not include applicable tax, shipping & handling, or other charges.

  6. UPstart™ Taq Antibody, 1 mg/ml

    UPstart™ Taq Antibody, 1 mg/ml

    Features
    • Inhibition of >95% Taq activity at 60°C
    • 200 ng UPstart Taq Antibody are blocking 1 U Taq DNA polymerase
    • Exceptionally pure— made in cell culture
    Applications
    • Thermolabile inhibition of Taq DNA polymerases
    • Convenient hot-start PCR setup at room temperature
    • Fast polymerase activation with the first PCR denaturation step
    Learn More…

    Package Sizes
    Starting at:
    €445.00

    Does not include applicable tax, shipping & handling, or other charges.

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