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Hot-Start PCR

biotechrabbit Hot-start PCR products include highly purified YourTaq™ Hot Start DNA Polymerase which is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Polymerase is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling direct PCR amplification without any DNA extraction. PCR mixes combine YourTaq, optimized buffer and high-quality dNTPs for the most demanding PCR applications. In addition, the enzyme is suitable for amplification of GC-rich templates (up to 70%) by using 5× PCR Enhancer. 

Convenient — master mixes including a direct-load version make your job easier
Specific and sensitive —  excellent performance for a broad range of amplicons
Efficient — abundant DNA even from low copy targets
Resistant — direct amplification from crude samples
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  1. YourTaq™ Hot Start PCR Mix, 2×

    YourTaq™ Hot Start PCR Mix, 2×

    Features
    • Exceptionally pure YourTaq Hot Start DNA Polymerase
    • Optimized Master Mix for increased yield of amplification
    • Resistant to PCR inhibitors for direct PCR amplification
    • Excellent PCR specificity and sensitivity for a broad range of amplicons
    Applications
    • Routine and demanding PCR amplification up to 3 kb
    • Suitable for amplification of low target copy number
    • TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €98.00

    Does not include applicable tax, shipping & handling, or other charges.

  2. YourTaq™ Direct-Load Hot Start PCR Mix, 2×

    YourTaq™ Direct-Load Hot Start PCR Mix, 2×

    Features
    • Exceptionally pure YourTaq Hot Start DNA Polymerase
    • Optimized Master Mix for increased yield of amplification and direct loading on the gel
    • Resistant to PCR inhibitors for direct PCR amplification
    • Excellent PCR specificity and sensitivity for a broad range of amplicons
    Applications
    • Routine and demanding PCR amplification up to 3 kb
    • Suitable for amplification of low target copy number
    • TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €102.00

    Does not include applicable tax, shipping & handling, or other charges.

  3. YourTaq™ Hot Start PCR Mix, 2×, lyophilized

    YourTaq™ Hot Start PCR Mix, 2×, lyophilized

    Features
    • Stable enzyme and mix for ambient shipment and room-temperature storage
    • Exceptionally pure YourTaq Hot Start DNA Polymerase
    • Optimized Master Mix for increased yield of amplification
    • Resistant to PCR inhibitors for direct PCR amplification
    • Excellent PCR specificity and sensitivity for a broad range of amplicons
    Applications
    • Routine and demanding PCR amplification up to 3 kb
    • Suitable for amplification of low target copy number
    • TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €38.00

    Does not include applicable tax, shipping & handling, or other charges.

  4. YourTaq™ Hot Start DNA Polymerase, 5 U/µl

    YourTaq™ Hot Start DNA Polymerase, 5 U/µl

    Features
    • Exceptionally pure YourTaq Hot Start DNA Polymerase
    • Optimized buffer composition for increased yield of amplification
    • Resistant to PCR inhibitors for direct PCR amplification
    • Excellent PCR specificity and sensitivity for a broad range of amplicons
    Applications
    • Routine and demanding PCR amplification up to 3 kb
    • Suitable for amplification of low target copy number
    • TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €52.00

    Does not include applicable tax, shipping & handling, or other charges.

  5. ApStarTaq™ DNA Polymerase, 5 U/µl

    ApStarTaq™ DNA Polymerase, 5 U/µl

    Features
    • Aptamer-based hot-start functionality
    • No activation steps needed
    • High PCR specificity and sensitivity
    • Exceptionally pure Taq DNA Polymerase for sensitive PCR applications and high yields
    Applications
    • Fast PCR reactions in endpoint and real-time analysis
    • Hot-start PCR up to 3 kb
    • Amplification of low-copy-number targets
    • RT-PCR and TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €52.00

    Does not include applicable tax, shipping & handling, or other charges.

  6. Hot Start Taq DNA Polymerase (wild-type), 5 U/µl

    Hot Start Taq DNA Polymerase (wild-type), 5 U/µl

    Features
    • High PCR specificity and sensitivity
    • Exceptionally pure Hot Start Taq DNA Polymerase for sensitive PCR applications and high yields
    • Antibody-based Hot Start for fast polymerase activation
    Applications
    • Hot-start PCR up to 3 kb
    • Amplification of low-copy-number targets
    • RT-PCR and TA cloning
    Learn More…

    Package Sizes
    Starting at:
    €80.00

    Does not include applicable tax, shipping & handling, or other charges.

  7. UPstart™ Taq Antibody, 1 mg/ml

    UPstart™ Taq Antibody, 1 mg/ml

    Features
    • Inhibition of >95% Taq activity at 60°C
    • 200 ng UPstart Taq Antibody are blocking 1 U Taq DNA polymerase
    • Exceptionally pure— made in cell culture
    Applications
    • Thermolabile inhibition of Taq DNA polymerases
    • Convenient hot-start PCR setup at room temperature
    • Fast polymerase activation with the first PCR denaturation step
    Learn More…

    Package Sizes
    Starting at:
    €445.00

    Does not include applicable tax, shipping & handling, or other charges.

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