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GenUP™ Plant RNA Kit

Features

  • Fast and simple procedure
  • High-quality RNA isolated from a wide variety of plant samples
  • Physical removal of DNA, no DNase treatment, no toxic β-mercaptoethanol

Applications

  • Isolation of total RNA from up to 100 mg plant material

Purchase GenUP™ Plant RNA Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0701501 10 preps Solutions, Mini Filters and Vials
€74.00
BR0701502 50 preps Solutions, Mini Filters and Vials
€246.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit™ GenUP Plant RNA Kit has been developed for quick and easy purification of total RNA from plant materials. After initial homogenization and lysis, genomic DNA is bound to a Mini Filter DNA, which can be discarded. RNA is selectively bound to a Mini Filter RNA, washed with two different buffers and eluted. The purified RNA is ready for use in any demanding molecular biology application, including RT-PCR.

Two lysis buffers, Buffer LYSIS LR and Buffer LYSIS LT, are provided to maximize yield. Most plant material can be processed with Buffer LYSIS LR. In the cases that yield using Buffer LYSIS LR is low, use Buffer LYSIS LT.


STARTING MATERIAL

Plant material (up to 100 mg)

EXTRACTION TIME

30 min after homogenization

BINDING CAPACITY

Approximately 100 µg RNA

TYPICAL YIELD

Variable depending on the starting material; approximately 70 µg RNA

AVERAGE PURITY

A260/A280 1.7–2.0


STEPS

  • Homogenize plant material and lyse with Buffer LYSIS LR or Buffer LYSIS LT.
  • Centrifuge to remove unlysed material.
  • Transfer the lysate to the Mini Filter DNA (blue).
  • Centrifuge, and discard the Mini Filter DNA.
  • The filtrate contains the RNA, do not discard.
  • Add ethanol to the filtrate.
  • Transfer to a Mini Filter RNA (violet) to bind RNA and centrifuge.
  • Add Buffer WASH A and centrifuge.
  • Add Buffer WASH B and centrifuge.
  • Centrifuge once more to remove residual ethanol.
  • Elute RNA with Water, RNase-free (for ELUTION), and centrifuge.
  • Purified RNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

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