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GenUP™ BS Virus RNA Kit

Features

  • Fast and simple procedure
  • High yields of pure RNA
  • No DNase treatment, no toxic β-mercaptoethanol

Applications

  • Virus RNA isolation from swabs

Purchase GenUP™ BS Virus RNA Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0702303 250 preps Solutions, Mini Filters and Vials
€894.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit™ GenUP BS Virus RNA Kit has been specially developed for quick and easy isolation of viral RNA from swabs, including nasopharyngeal and oropharyngeal swabs. The unique binding membrane of our high-capacity Mini Filters guaranties high yields. A high concentration of purified RNA can be achieved with flexible elution volumes. The kit includes carrier RNA.

After few initial steps, the viral RNA is bound to a Mini Filter, washed, and then eluted in a separate tube. The purified RNA is ready to be used in all demanding molecular biology applications, including qRT-PCR.


STARTING MATERIAL

Swabs samples

EXTRACTION TIME

Approximately 25 min

TYPICAL YIELD

Yield is dependent on sample quality and viral load


STEPS

  • Extract swab and lyse with Proteinase K.
  • Centrifuge.
  • Add isopropanol, mix and transfer to a Mini Filter.
  • Centrifuge.
  • Add Buffer WASH B and centrifuge.
  • Wash 2 times with 80% ethanol and centrifuge.
  • Centrifuge once more to remove residual ethanol.
  • Add pre-heated Water, RNase-free, incubate and centrifuge.
  • RNA is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

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