biotechrabbit™ ExactiFi™ Hot-Start DNA Polymerase is a highly purified thermostable recombinant proofreading DNA polymerase. ExactiFi exhibits approximately 50 times higher accuracy than Taq DNA polymerase and amplifies targets up to 5–10 kb in size.
The enzyme catalyzes template-dependent nucleotide polymerization in the 5'→3' direction. Additionally, the 3'→5' exonuclease (proofreading) activity corrects nucleotide incorporation errors, thereby increasing fidelity and accuracy of DNA polymerization. The enzyme has no 5'→3' exonuclease activity and no detectable reverse transcriptase activity, producing blunt-end PCR products.
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