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RTS 100 Wheat Germ Kit

Features

  • Efficient expression of up to 5 μg protein per reaction
  • Compatible with non-optimized, pro- and eukaryotic T7 promoter-based vectors as well as linear templates
  • Excellent homogeneity of large proteins (264 kDa expressed)
  • No endotoxins, allows direct use in cell-based assays
  • Upscaling using high yield CECF wheat germ kits

Applications

  • Fast parallel synthesis for screening purposes
  • Synthesis of mammalian or other proteins for functional testing without the disturbing endogenous protein background that is typical for in vivo expression
  • Synthesis of proteins dependent on eukaryotic chaperones

Purchase RTS 100 Wheat Germ Kit

Availability: In stock Bulk requests please inquire at oem@biotechrabbit.com

Catalog # Size Package Content Price* Qty
BR1402501 24 rxn of 50 µl Reagents for coupled transcription/translation reactions
€581.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

The biotechrabbit RTS 100 Wheat Germ Kit is a batch screening system allowing rapid protein expression within 2.5 hours. The kit allows use of a broad range of nonoptimized T7 promoter-based expression vectors from prokaryotic and eukaryotic origin in addition to established cell-free wheat germ vectors or linear templates. Using this approach, multiple constructs — including vectors that have not been optimized for wheat germ — can be screened rapidly for expression and functionality in parallel. The procedure saves precious time otherwise required for template generation. The new batch system yields up to 100 μg protein per ml reaction, which is sufficient for many downstream applications.

 

Proteins expressed

Standard soluble proteins
Complex and large proteins
Membrane proteins

Reaction time / yield

3 h / 5 μg

Reaction mode

Batch

Template

RTS Wheat Germ LinTempGen Set
RTS pIVEX Wheat Germ His6-tag Vector


Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

  • Component testing: each component is tested to ensure the composition and quality meet stated specifications.
  • Performance testing: each product is tested to ensure it meets the stated performance specification.


The RTS 100 Wheat Germ Kit enables high success rates for expression and solubility, particularly for eukaryotic target proteins. Synthesize proteins in batch format within 2.5 hours using a broad range of non-optimized T7 promoter-based expression vectors of pro- and eukaryotic origin. Convenience is greatly improved, due to the ability to use PCR-generated linear templates in addition to plasmid templates. Use this kit to synthesize proteins in a wide molecular weight range (up to 264 kDa tested), as well as to synthesize truncated protein variants (e.g., for epitope or functional domain mapping).

For detailed protocol, please, refer to the product manual (Related Documents).

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