biotechrabbit™ 2X RT-RPA Basic Master Mix, lyophilized is a robust and versatile kit for rapid RNA amplification under isothermal conditions. This ready-to-use, freeze-dried master mix is designed to enable sensitive, specific, and efficient RNA amplification at a constant temperature of 37°C to 42°C, avoiding the need for a thermal cycler. Formulated as a 2X master mix, it simplifies reaction setup by combining all essential enzymatic and buffer components into a single, easily reconstituted pellet.

The mix contains a carefully optimized combination of reverse transcriptase, recombinase, strand-displacing DNA polymerase, single-stranded DNA-binding proteins, and cofactors, ensuring rapid strand invasion and extension from primer-bound RNA targets. Its lyophilized format guarantees room-temperature stability and long shelf life, while maintaining consistent performance across a wide range of applications. This stability allows for seamless integration into workflows where refrigeration or cold chain logistics are limited or unavailable.

Produced under strict quality control standards, the 2X RT-RPA Basic Master Mix is thoroughly tested for activity, reproducibility, and the absence of contaminating nucleic acids or nucleases. This ensures accurate, low-background amplification and reproducible results, even with low-copy-number RNA targets. The high purity of the mix minimizes the risk of nonspecific amplification, enabling reliable detection of specific sequences in complex samples.

The 2X RT-RPA Basic Master Mix, lyophilized, is ideally suited for a variety of molecular biology workflows, including research on pathogen detection, genetic screening, and synthetic biology applications that require fast, precise RNA amplification under constant-temperature conditions. Its speed, stability, and convenience make it a valuable tool for modern nucleic acid amplification needs.