biotechrabbit™ MgCl2 is ideally suited for optimization of PCR conditions. For convenience and flexibility, it is provided as 50 mM solution.
Component | Composition |
50 mM MgCl2 | PCR Grade 50 mM MgCl2 in water |
STORAGE | -20°C (until expiry date – see product label) |
Quality Control
Functional Assay
Human genomic DNA was amplified using Taq DNA Polymerase and specific primers to produce a distinct band of 750 bp.
Optimizing magnesium concentration
Many applications use the standard concentration of 1.5 mM MgCl2. However, reactions with increased amounts of template (e.g., genomic DNA), primer and nucleotides might require higher MgCl2 concentrations (2–3 mM). The 50 mM MgCl2 solution can be used to adjust the MgCl2 concentration according to the table below:
Final concentration of MgCl2 in a 50 µl reaction, mM | 1.5 | 1.75 | 2.0 | 2.5 | 3.0 |
Volume of 50 mM MgCl2 solution to add, µl | 1.5 | 1.75 | 2.0 | 2.5 | 3.0 |
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