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GenUP™ Bacteria gDNA Kit

Features

  • Fast and simple procedure
  • gDNA from up to 1 × 109 bacteria
  • High yields of pure DNA for demanding applications

Applications

  • Isolation of bacterial genomic DNA from Gram-positive and Gram-negative bacteria

Purchase GenUP™ Bacteria gDNA Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0700701 10 preps Solutions, Mini Filters and Vials
€45.00
BR0700702 50 preps Solutions, Mini Filters and Vials
€148.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit™ GenUP Bacterial gDNA Kit has been specially developed for quick and easy purification of bacterial genomic DNA from both  Gram-negative and difficult to process Gram-positive bacteria.

A combined lysozyme and proteolytic lysis steps allow efficient cell disruption. The DNA is bound to a high-capacity filter, washed and then eluted in a separate tube. The purified DNA is ready to be used in all demanding molecular biology applications, including PCR, enzymatic digestions, cloning and other.


STARTING MATERIAL

1 × 109 Gram-negative or Gram-positive bacterial cells

EXTRACTION TIME

Approximately 45 min

BINDING CAPACITY

> 50 µg DNA

TYPICAL YIELD

Variable; approximately 35 µg


STEPS

  • Pellet 5.0–15.0 ml bacterial culture and centrifuge. Remove the supernatant.
  • Resuspend the cell pellet in TE buffer.
  • Add lysozyme and incubate.
  • Add Buffer LYSIS LB and Proteinase K, mix and incubate.
  • Centrifuge to pellet unlysed material
  • Transfer the lysate to a new tube and add Buffer BINDING BG.
  • Apply the mixture to a Mini Filter and centrifuge.
  • Wash with Buffer WASH A and Buffer WASH C and centrifuge.
  • Centrifuge again to remove residual ethanol.
  • Elute DNA in Buffer ELUTION P.
  • Purified DNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

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