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RTS 100 E. coli HY Kit

Features

  • Compatible with linear templates for fast screening without cloning
  • Convenient reaction setup
  • Fast expression of proteins in parallel
  • Improved high yield biochemistry for up to 20 μg protein per reaction

Applications

  • Fast parallel microgram-scale protein expression

Purchase RTS 100 E. coli HY Kit

Availability: In stock Bulk requests please inquire at oem@biotechrabbit.com

Catalog # Size Package Content Price* Qty
BR1400106 6 rxn of 50 µl Reagents for coupled transcription/translation reactions
€174.00
BR1400101 24 rxn of 50 µl Reagents for coupled transcription/translation reactions
€525.00
BR1400102 96 rxn of 50 µl Reagents for coupled transcription/translation reactions
€1,659.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

The biotechrabbit RTS 100 E. coli HY Kit contains a modified high-yield E. coli lysate to drive coupled in vitro transcription and translation reactions. The enhanced kit can express up to 20 μg protein (50 μl reaction) in 4 hours using a batch mode. The RTS 100 E. coli HY kit is compatible with both linear templates and plasmids, making it the product of choice for protein-expression screening and optimization.

Each kit contains everything you need to perform protein-expression in the delivered microplate or, alternatively, in microtubes.

(former product codes: 24 rxn kit RiNA N-1101-24, 5PRIME 2401100 / 96 rxn kit RiNA N-1101-96, 5PRIME 2401110)

 

Proteins expressed

Standard soluble proteins
Membrane proteins

Reaction time / yield

1–4 h / 20 μg

Reaction mode

Batch

Template

RTS Linear Template Kit Plus
RTS Linear Template Fab Kit
RTS 100 E. coli LinTempGen Set, His
RTS pIX3.0 Vector
RTS pIVEX E. coli His-tag, 2nd Gen. V.


Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

  • Component testing: each component is tested to ensure the composition and quality meet stated specifications.
  • Performance testing: each product is tested to ensure it meets the stated performance specification.


The RTS 100 E. coli HY Kit is designed to turn genes into proteins within several hours, producing microgram yields to facilitate the assignment of biological functions to genes. Protein expression conditions can be optimized to enhance active protein production by adding chaperones, detergents, or other reagents to the reaction. The microplate format is easily adapted to high-throughput protein expression.

For detailed protocol, please, refer to the product manual (Related Documents).

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