biotechrabbit™ 2X RPA Exo Master Mix, Lyophilized is a high-performance reagent system for rapid, real-time DNA amplification under isothermal conditions. Formulated as a 2X lyophilized master mix, it enables fast, sensitive, and specific detection of DNA targets at a constant temperature of 37°C to 42°C—without the need for thermal cycling. The mix is designed for fluorescence-based detection using exonuclease III-cleavable probes, providing quantitative results within minutes. This all-in-one master mix contains a carefully balanced blend of recombinase, strand-displacing DNA polymerase, single-stranded DNA-binding proteins, exonuclease III, and optimized cofactors. Upon reconstitution, it supports efficient primer invasion, strand displacement, and probe cleavage during amplification. The use of exonuclease probes allows the generation of real-time fluorescence signals, ensuring precise and dynamic monitoring of amplification as it occurs.

The lyophilized format guarantees room-temperature stability and extended shelf life, while reducing handling steps and minimizing the risk of pipetting errors. Reconstitution with the provided buffer yields a fully active master mix that is ready for immediate use in quantitative DNA detection workflows.

Manufactured under stringent quality control conditions, the 2X RPA Exo Master Mix is tested for consistent performance, enzymatic activity, and the absence of contaminating nucleic acids or nucleases. This ensures highly specific, low-background amplification and reproducible real-time detection, even in demanding applications.

The 2X RPA Exo Master Mix, Lyophilized, is ideally suited for quantitative isothermal assays, molecular diagnostics development, and rapid screening workflows requiring fast, reliable, and fluorescence-based DNA detection in a temperature-stable, easy-to-use format.