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GenUP™ gDNA Kit

Features

  • Universal kit for DNA isolation from various starting materials
  • Fast and simple procedure with > 100 µg gDNA filter-binding capacity
  • High yields of pure DNA

Applications

  • Isolation of genomic DNA from tissues, cells, rodent tail, buccal swaps and paraffin samples

Purchase GenUP™ gDNA Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0700601 10 preps Solutions, Mini Filters and Vials
€42.00
BR0700602 50 preps Solutions, Mini Filters and Vials
€139.00
BR0700603 250 preps Solutions, Mini Filters and Vials
€570.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit GenUP™ gDNA Kit is designed for a fast and efficient genomic DNA isolation from various sources and different amounts of starting material, such as mammalian tissues (including paraffin-embedded), buccal swabs and eukaryotic cell cultures. After a few initial procedures, the genomic DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. High yields of pure, unshared genomic DNA are ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.


STARTING MATERIAL

Tissue samples (up to 40 mg), rodent tail specimens of 0.5–1 cm in length, eukaryotic cells (up to 5 × 106), formalin-fixed paraffin-embedded tissue samples (FFPE, 2–4 slices), buccal swabs

EXTRACTION TIME

Approximately 8 min after lysis

BINDING CAPACITY

> 100 µg DNA

TYPICAL YIELD

Variable; approximately 65 µg

AVERAGE PURITY

A260/A280 1.7–2.0


STEPS

  • Collect the sample material and perform lysis.
  • Centrifuge to pellet unlysed material.
  • Transfer the supernatant to a new tube and add Buffer BINDING BD.
  •  Apply the mixture to a Mini Filter (blue) and centrifuge.
  • Wash the DNA twice with Buffer WASH C and centrifuge.
  • Centrifuge again to remove residual ethanol.
  • Elute DNA with Buffer ELUTION and centrifuge.
  • Purified genomic DNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

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