Shopping Cart - €0.00

You have no items in your shopping cart.

Quick Order
Return to Previous Page

RTS pIX3.0 Vector

Features

  • Multiple cloning site compatible with linear templates generated with RTS Linear Template Kit Plus and RTS Linear Template Fab Kit
  • High copy origin of replication
  • Cell-free protein synthesis scale-up to after expression screening using linear templates
  • Free of any potentially interfering regulatory elements ensuring efficient transcription and translation
  • Compatible with RTS E. coli and insect kits

Applications

  • Cloning of RTS Linear Templates for expression in RTS E. coli and insect kits

Purchase RTS pIX3.0 Vector

Availability: In stock Bulk requests please inquire at oem@biotechrabbit.com

Catalog # Size Package Content Price* Qty
BR1402701 1 vector 25 µg pIX3.0 Vector
€356.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

The cloning vector RTS pIX3.0 is designed for cloning linear templates generated with RTS Linear Template Plus and Fab kits to subsequently up-scale protein synthesis in E. coli batch systems to the CECF format or to use with RTS insect kits. The vector contains a multiple cloning site compatible with that of the linear templates.

The RTS pIX3.0 Vector enables high-level protein expression using the RTS 100/500/9000 E. coli HY HY kits, E. coli Disulfide and RTS Insect kits. The kit exhibits the same performance as the RTS pIVEX His-tag, 2nd Gen. Vector Set, which is used for classic cloning.

(former FastLane product codes: Vector RiNA T-1103-01)

 

Protein tag

Tag from linear template

Expression kits

RTS 100 E. coli HY Kit
RTS 500 ProteoMaster E. coli HY Kit
RTS 9000 E. coli HY Kit
RTS 100 E. coli Fab Kit
RTS 100 E. coli Disulfide Kit
RTS 500 E. coli Disulfide Kit
RTS 100 Insect Membrane Kit
RTS 100 Site-Specific Label Kit


Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

  • Component testing: each component is tested to ensure the composition and quality meet stated specifications.
  • Performance testing: each product is tested to ensure it meets the stated performance specification.


The RTS pIX3.0 Vector is used for upscaling protein synthesis with RTS E. coli-based and Insect-based kits after expression screening using PCR-generated Linear Templates. These PCR products generated with RTS Linear Template Kit Plus and RTS Linear Template Fab Kit contain multiple cloning sites that are compatible for cloning into the RTS pIX3.0 Vector.
The resulting expression plasmid is added to an upscaled in vitro transcription/translation reaction mix and incubated. After incubation, the proteins can be purified and analyzed, e.g., by SDS-PAGE or functional assays.

For detailed protocol, please, refer to the product manual (Related Documents).

You may also be interested in the following product(s)
RTS 100 E. coli HY Kit

RTS 100 E. coli HY Kit

Package Sizes
Starting at:
€215.00

dNTP Mixes

dNTP Mixes

Package Sizes
Starting at:
€70.00

RTS 500 ProteoMaster E. coli HY Kit

RTS 500 ProteoMaster E. coli HY Kit

Package Sizes
Starting at:
€2,535.00

RTS GroE Supplement

RTS GroE Supplement

Package Sizes
Starting at:
€366.00

cDNA Synthesis Kit

cDNA Synthesis Kit

Package Sizes
Starting at:
€64.00