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RTS 9000 E. coli HY Kit

Features

  • Unique CECF technology to ensure high yields
  • Expression of toxic proteins that cannot be produced in vivo
  • Incorporation of labeled methionine into proteins

Applications

  • Large-scale, cell-free expression of up to 50 mg protein

Purchase RTS 9000 E. coli HY Kit

Availability: In stock Bulk requests please inquire at oem@biotechrabbit.com

Catalog # Size Package Content Price* Qty
BR1400301 1 reactions of 10 ml Reagents for coupled transcription/translation reactions
€4,537.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

One reaction of the biotechrabbit RTS 9000 E. coli HY Kit yields ten times more total protein (up to 50 mg) than an RTS 500 reaction. The kit provides a reaction volume ten times higher than the RTS 500 E. coli HY Kit, while maintaining a similar productivity per milliliter of lysate.

The RTS 9000 Reaction Device is composed of a reaction compartment and a feeding compartment. The 10 ml reaction compartment, where the coupled transcription/ translation reactions take place, is separated from the 100 ml feeding compartment by a semipermeable membrane (cut-off 10 kDa).

(former product codes: RiNA N-1205-10, 5PRIME 2401900)

 

Proteins expressed

Standard soluble proteins
Membrane proteins

Reaction time / yield

6–24 h / 50 mg

Reaction mode

CECF

Template

RTS pIX3.0 Vector
RTS pIVEX E. coli His-tag, 2nd Gen. V.


Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

  • Component testing: each component is tested to ensure the composition and quality meet stated specifications.
  • Performance testing: each product is tested to ensure it meets the stated performance specification.


Due to the production of preparative-levels of protein, sufficient amounts for various downstream applications can be obtained using the RTS 9000 E. coli HY Kit. It is also suitable for the production of protein for NMR spectroscopy or X-ray crystallography. The kit design allows convenient and easy labeling of proteins by exchanging the provided amino acids with amino acids mixtures of your choice. Methionine (included in the kit) can be easily exchanged for seleno-methionine (not included). Other amino acids can be exchanged by preparing tailored amino-acid mixtures.

For detailed protocol, please, refer to the product manual (Related Documents).

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