biotechrabbit™ GenUP Plasmid Kit is designed for fast and efficient plasmid purification from 0.5–15 ml bacterial suspensions in a mini format. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, plasmid DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. Typically, yields are up to 60 µg plasmid DNA from a 15 ml bacterial suspension, depending on a plasmid copy number.
The purified plasmid DNA is ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.
STARTING MATERIAL | Bacterial cultures: 0.5–15 ml for high-copy-number plasmids, low-copy-number plasmids or cosmids |
EXTRACTION TIME | Approximately 15 min |
BINDING CAPACITY | Approximately 80 µg plasmid DNA |
TYPICAL YIELD | Variable: 45–60 µg high-copy plasmid DNA is typically purified from 15 ml culture, or 6–20 µg from 2 ml culture |
AVERAGE PURITY | A260/A280 1.7–2.0 |
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For detailed protocol, please, refer to the product manual (Related Documents).
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