Hot-Start PCR
biotechrabbit Hot-Start PCR products include highly purified YourTaq™ Hot-Start DNA Polymerase which is optimized for high yield of amplification of up to 5 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Polymerase is resistant to PCR inhibitors, such as blood (up to 20 %), ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors. PCR mixes combine YourTaq, optimized buffer and high-quality dNTPs for the most demanding PCR applications. In addition, the enzyme is suitable for amplification of GC-rich templates (up to 70 %) by using 5X PCR Enhancer.
Convenient — master mixes including a direct-load version make your job easier
Specific and sensitive — excellent performance for a broad range of amplicons
Efficient — abundant DNA even from low copy targets
Resistant — direct amplification from crude samples
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ExactiFi™ Hot-Start DNA Polymerase, 1 U/µl
Features
- Exceptional fidelity, approximately 50 times higher than Taq polymerase
- Amplifies targets up to 5–10 kb in size
- Produces blunt-end PCR products
- Efficient amplification of both low- and high-complexity targets
- High resistance to inhibitors for robust performance across a variety of sample types
Applications
- High-fidelity PCR
- Generation of PCR products for blunt cloning
Package Sizes
Starting at: €85.00Does not include applicable tax, shipping & handling, or other charges.