biotechrabbit™ GenUP Bacterial gDNA Kit has been specially developed for quick and easy purification of bacterial genomic DNA from both Gram-negative and difficult to process Gram-positive bacteria.
A combined lysozyme and proteolytic lysis steps allow efficient cell disruption. The DNA is bound to a high-capacity filter, washed and then eluted in a separate tube. The purified DNA is ready to be used in all demanding molecular biology applications, including PCR, enzymatic digestions, cloning and other.
STARTING MATERIAL | 1 × 109 Gram-negative or Gram-positive bacterial cells |
EXTRACTION TIME | Approximately 45 min |
BINDING CAPACITY | > 50 µg DNA |
TYPICAL YIELD | Variable; approximately 35 µg |
STEPS |
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For detailed protocol, please, refer to the product manual (Related Documents).