biotechrabbit™ GenUP Total RNA Kit has been specially developed for a quick and easy purification of total RNA from eukaryotic cell suspensions, tissues and biopsies, Gram-negative (e.g., E. coli) and Gram-positive bacteria and other sources. After few initial procedures, the RNA is bound to a filter, washed and then eluted in a separate tube. DNA is removed physically by binding to a filter without any DNAse treatment or the use of toxic β‑mercaptoethanol. The purified RNA is ready to be used in all demanding molecular biology applications, including cDNA synthesis, northern blot analysis and others.
STARTING MATERIAL | Eukaryotic cells (5 × 106), tissue samples (up to 20 mg), bacterial cells |
EXTRACTION TIME | Approximately 20–40 min |
BINDING CAPACITY | 100 µg RNA |
TYPICAL YIELD | Yield is highly dependent on sample type |
STEPS |
|
|
|
|
|
For detailed protocol, please, refer to the product manual (Related Documents).
You may also be interested in the following product(s)
2X YourTaq™ PCR Master Mix
Package Sizes |
RTS 100 Insect Membrane Kit
Package Sizes |
RTS pIVEX Wheat Germ His6-tag Vector Set
Package Sizes |
2X Pfu PCR Master Mix
Package Sizes |
2X RPA basic Master Mix, lyophilized
Package Sizes |