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Real Time PCR

biotechrabbit QPCR Green, Probe and One-Step QRTPCR Master Mixes are first-choice products for fast and easy real-time PCR setup. They contain all reagents required for real-time PCR and are designed to achieve excellent results in reaction efficiency, correlation coefficient and slope.
Intercalating-dye-based mixes, such as QPCR Green Master Mix, allow nonspecific detection of double-stranded DNA and are faster and more cost-effective than probebased mixes. Probe-based mixes, such as QPCR Probe Master Mix, can be used specifically or universally. However, more effort is required to design and synthesized specific probes. biotechrabbit QPCR and QRT-PCR products can be used with the fast and standard modes as well as for multiplex PCR. The mixes are available with passive reference dyes Rox™ (carboxy-X-rhodamine) or fluorescein to enable the correction of errors caused by the instrument.

High performance — rapid extension rate for early Ct values
Sensitive — increased limit of detection for quicker results
Compatible — use on any real-time PCR platform
Flexible — for standard and fast cycling consitions

 

 

Instrument compatibility and recommendation for passive reference dyes

Reference dye

Company

Instrument

Low concentration ROX

Analytica Jena
Applied Biosystems
Bio-Rad

Cepheid
Eppendorf
Illumina
QIAGEN
Roche Applied Science
Agilent
Takara
Techne

qTower
7500, 7500 FAST, Viia™ 7
iCycler®, MyiQ™, iQ™ 5, Opticon™, Opticon 2, Chromo4™, MiniOpticon™ , CFX96™, CFX384™
Smartcycler®
Mastercycler® ep REALPLEX®, Mastercycler REALPLEX 2S Eco™
Rotor-Gene® 3000, 6000, Q
Lightcycler® 480, Lightcycler Nano
Mx4000P®, Mx3000P®, Mx3005P®
Cycler Dice™
Quantica®

High concentration ROX

Analytica Jena
Applied Biosystems

Cepheid
Eppendorf
Illumina
QIAGEN
Roche Applied Science
Takara
Techne

qTower
7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOnePlus™
Smartcycler
Mastercycler ep REALPLEX, Mastercycler REALPLEX 2S
Eco
Rotor-Gene 3000, 6000, Q
Lightcycler 480, Lightcycler Nano
Cycler Dice
Quantica

Fluorescein


Bio-Rad


iCycler, MyiQ, iQ 5