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RTS Linear Template Fab Kit

Features

  • Introduction of an affinity tag for protein purification
  • Signal peptide sequence for translocation into microsomes
  • Introduction of a signal peptide sequence for translocation into microsomes
  • Compatibility with E. coli HY, Fab and Insect kits

Applications

  • PCR-based inear templates for protein expression in E. coli and insect disulfide kits
  • Fast template generation for expression of Fab and scFv antibody fragments

Purchase RTS Linear Template Fab Kit

Availability: In stock Bulk requests please inquire at oem@biotechrabbit.com

Catalog # Size Package Content Price* Qty
BR1402201 96 PCRs of 25 µl PCR reagents for linear template generation
€283.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

The RTS Linear Template Fab Kit is designed for rapid PCR-based generation of templates directly used in the E. coli Fab and Insect kits allowing functional screening of antibody fragments and disulfide proteins and to facilitate the assignment of biological functions to genes. The templates can be generated with optional affinity tag and/ or signal peptide sequences for protein purification and translocation into microsomes.

The RTS Linear Template Fab Kit generates templates with elements to drive efficient coupled in vitro transcription and translation reactions for the synthesis of proteins with intra- and intermolecular disulfide bonds. The kit is the product of choice for functional screening of disulfide proteins and optimization of constructs. One kit contains sufficient volumes of primer solutions to perform 96 standard protein expression reactions with reaction volumes of 25 μl.

(former FastLane product codes: RiNA C-1217-96)

 

Protein tag

His-tag
Signal peptide

Expression kits

RTS 100 E. coli HY Kit
RTS 100 E. coli Fab Kit
RTS 100 Insect Membrane Kit
RTS 100 Site-Specific Label Kit


Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

  • Component testing: each component is tested to ensure the composition and quality meet stated specifications.
  • Performance testing: each product is tested to ensure it meets the stated performance specification.


Use the RTS Linear Template Fab Kit (96) for the synthesis of templates encoding Fab, scFv and other disulfide proteins.
 
It is designed to turn open reading frames into expressable templates, which can be directly used in RTS 100 E. coli Fab kits, RTS 100 Insect Disulfide kits and RTS 100 E. coli HY kits.

Optionally provide your templates with affinity tag or signal peptide sequences for protein purification and translocation into microsomes.

For detailed protocol, please, refer to the product manual (Related Documents).

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