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GenUP™ Micro RNA Kit

Features

  • Fast and simple procedure
  • Optimized binding conditions for high yields of small RNAs
  • Physical removal of DNA, no DNase treatment, no toxic β-mercaptoethanol

Applications

  • Efficient isolation of snRNA, miRNA, siRNA, tRNA, rRNA and mRNA from various starting material

Purchase GenUP™ Micro RNA Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0701901 10 preps Solutions, Mini Filters and Vials
€71.00
BR0701902 50 preps Solutions, Mini Filters and Vials
€235.00
BR0701903 250 preps Solutions, Mini Filters and Vials
€964.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit™ GenUP Micro RNA Kit is designed for high yields of small RNA molecules and total RNA, including snRNA, miRNA, siRNA, tRNA, rRNA and mRNA, without the use of highly toxic β-mercaptoethanol. After using well established filter-technology to selectively remove genomic DNA, the RNA is bound, washed and eluted from the filter membrane using RNase-free water.


STARTING MATERIAL

Eukaryotic cells (5 × 106)
Fresh or frozen tissue or biopsies (up to 20 mg)
Gram-positive and Gram-negative bacteria (up to 1 × 109)

EXTRACTION TIME

Typically 15–40 minutes

BINDING CAPACITY

100 µg

Yield

Depends on the type and the amount of the starting material

RECOVERY RATE

High rate of recovery for small RNA molecules


STEPS

  • Homogenize and lyse the sample material.
  • Centrifuge to pellet unlysed material.
  • Transfer the lysate to the Mini Filter DNA (blue).
  • Centrifuge, and discard the Mini Filter DNA with bound DNA.
  • The filtrate contains the RNA, don’t discard!
  • Add isopropanol to the filtrate.
  • Transfer to a Mini Filter RNA (violet) to bind RNA and centrifuge.
  • Add Buffer WASH A and centrifuge.
  • Add Buffer WASH B and centrifuge.
  • Centrifuge once more to remove residual ethanol.
  • Add Water, RNase-free (for ELUTION), incubate and centrifuge.
  • microRNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

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