Shopping Cart - €0.00

You have no items in your shopping cart.

Quick Order
Return to Previous Page

4X CAPITAL™ qPCR Probe Master Mix, lyophilized


  • Stable enzyme and mix for ambient shipment and room-temperature storage
  • Best in-class performance for both single and multiplex detection
  • Convenient master mix with high specificity in pathogen detection
  • Highly sensitive for low-abundance DNA targets


  • Standard and fast cycling qPCR with rapid extension rate for early Ct values
  • For use on a wide range of probe technologies including Taqman®, Molecular Beacons® and Scorpion® probes

Purchase 4X CAPITAL™ qPCR Probe Master Mix, lyophilized

Availability: In stock Bulk requests please inquire at

Catalog # Size Package Content Price* Qty
BR0502601 200 reactions of 20 µl 2 × Lyo CAPITAL qPCR Probe Master Mix
2 × qPCR Probe Reconstitution Buffer

*Does not include applicable tax, shipping & handling, or other charges.


biotechrabbit™ lyophilized CAPITAL qPCR Probe Mix is a freeze-dried version of the well-established liquid equivalent. The stabilized format allows shipment and storage without cooling. The master mix is optimized for quantifying genomic, cDNA and viral sequences provides outstanding performance in single and multiplex qPCR. The high sensitivity provided by the mix is ideal for detection of low-abundance DNA targets in various applications, such as pathogen detection. CAPITAL qPCR Probe Mix uses proprietary combination of enzyme and buffer chemistry for efficient extension and early Ct in single and multiplex qPCR.

Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).




Lyo CAPITAL qPCR Probe Mix

Cake of lyophilized 4× qPCR Probe Master Mix

qPCR Probe Reconstitution Buffer

Optimized PCR buffer for reconstituting lyophilized CAPITAL qPCR Probe Mix



1) Transfer the whole content of one vial qPCR Mix Reconstitution Buffer to one vial Lyo CAPITAL qPCR Probe Mix
2) Mix well – the lyophilisate will dissolve within seconds
3) Store the reconstituted CAPITAL qPCR Probe Mix at -20°C 


Store at room temperature or below (until expiry date – see product label)
Reconstituted lyophilisate: store at −20°C for up to 12 months



Quality Control
Functional assay

Mix tested functionally in qPCR.

  • For efficient amplification under fast cycling conditions use amplicon lengths between 80 bp and 200 bp.
  • The shorter the amplicon length the faster the reaction can be cycled.
  • Amplicon lengths should not exceed 400 bp.
  • Primers should have a predicted melting temperature of around 60°C, using default Primer 3 settings (
  • For TaqMan® probes choose probe close to 5’ primer, avoid terminal guanosine residues.
Prevention of PCR contamination

When assembling the amplification reactions, care should be taken to eliminate the possibility of contamination with undesired DNA.

  • Use separate clean areas for preparation of samples and reaction mixtures and for cycling.
  • Wear fresh gloves. Use sterile tubes and pipette tips with aerosol filters for PCR setup.
  • Use only water and reagents that are free of DNA and nucleases.
  • With every PCR setup, perform a contamination control reaction that does not include template DNA.
Basic Protocol
  • Keep the master mix protected from light until you use it.
  • Aliquot the master mix to minimize freeze-thaw cycles and light exposure.
  • Thaw on ice and mix very well all reagents. Assemble and keep all reactions on ice.
  • Use only high quality optically clear reaction plates and seals designed for fluorescence applications.
  • Do not use corner wells or use a more robust seal.
  • Reserve plate positions for positive (control DNA) and negative (water or buffer) controls.
  • First pipette the primer mixture, then add the template and last the Master Mix.
  • Before preparing mixes, calculate the volume needed according to the reaction number plus one extra.
  • To have a better correlation, run the reactions in triplets.



Final concentration

Primer Mix (Reverse and Forward)


100–400 nM

Too high primer concentrations result in unspecific amplification and should be avoided.

Specific Probe


200 nM

Template DNA


10 pg – 100 ng

Use diluted or undiluted cDNA from less than 1 µg RNA

CAPITAL qPCR Probe Mix, 4×
(reconstituted lyophilisate)

5 µl

Nuclease free water



Total volume

20 µl


  • Gently mix the reactions without creating bubbles (do not vortex). Bubbles will interfere with fluorescence detection.
  • Place the reaction into the PCR cycler.
Cycling Program





Initial activation


2-3 min




10–15 s




30 s

*Recommended annealing/extension temperature is primer Tm +2°C. Use gradient PCR to optimize the annealing temperature. Do not use temperatures below 60°C. Do not exceed 30 seconds.
For melt analysis refer to instrument instructions. 
You may also be interested in the following product(s)
GenUP™ FFPE Paraffin Removal Solution

GenUP™ FFPE Paraffin Removal Solution

Package Sizes
Starting at:

RTS Linear Template Fab Kit

RTS Linear Template Fab Kit

Package Sizes
Starting at:

2X Multiplex Hot-Start PCR Master Mix

2X Multiplex Hot-Start PCR Master Mix

Package Sizes
Starting at:

YourTaq™ Hot-Start DNA Polymerase, 5 U/µl

YourTaq™ Hot-Start DNA Polymerase, 5 U/µl

Package Sizes
Starting at:

Monoclonal Mouse Antibodies

Monoclonal Mouse Antibodies

Package Sizes
Starting at: