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RTS 100 Wheat Germ CECF Kit

Features

  • Successful expression of eukaryotic proteins , generally without sequence optimization
  • Synthesis of up to 50 µg functional eukaryotic protein
  • Maximized production of active heat-sensitive proteins, due to an optimum reaction temperature of 24°C

Applications

  • Up to milligram-scale protein expression of functional eukaryotic proteins
  • Expression of multiple proteins in parallel

Purchase RTS 100 Wheat Germ CECF Kit

Availability: In stock Bulk requests please inquire at oem@biotechrabbit.com

Catalog # Size Package Content Price* Qty
BR1401001 24 rxn of 50 µl Reagents for coupled transcription/translation reactions
€728.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

The biotechrabbit RTS 100 Wheat Germ CECF Kit enables high success rates for expression and solubility, particularly for eukaryotic target proteins. The use of PCR-generated linear templates greatly improves convenience. The kits allow synthesis of proteins in a wide molecular weight range (up to 220 kDa, as tested), as well as synthesis of truncated protein variants (e.g., for epitope or functional domain mapping). While the RTS 100 Wheat Germ CECF Kit is designed for high-throughput protein expression, the RTS 500 version ensures higher yields.

The system can be used with linear templates as well as mRNA, vectors and plasmid DNA. Depending on the protein, expression yields of more than 1 mg protein per reaction can be obtained within 24 hours in a 1 ml reaction.

(former product codes: RiNA N-2201-24, 5PRIME 2402100)

 

Proteins expressed

Standard soluble proteins
Complex and large proteins
Membrane proteins

Reaction time / yield

6–24 h / 50 μg (50 μl)

Reaction mode

CECF

Template

RTS Wheat Germ LinTempGen Set
RTS pIVEX Wheat Germn His6-tag Vect.


Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

  • Component testing: each component is tested to ensure the composition and quality meet stated specifications.
  • Performance testing: each product is tested to ensure it meets the stated performance specification.


The RTS 100 Wheat Germ CECF Kit enables high success rates for expression and solubility, particularly for eukaryotic target proteins. Convenience is greatly improved, due to the ability to use PCR-generated linear templates; plasmids and mRNA can also be used as template. Use this kit to synthesize proteins in a wide molecular weight range, as well as to synthesize truncated protein variants (e.g., for epitope or functional domain mapping). The microplate format is easily adapted to high-throughput protein expression and makes this an ideal kit for screening applications.

For detailed protocol, please, refer to the product manual (Related Documents).

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