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Cloning related applications

Endpoint PCR Technique Selection Guide for Cloning Applications
Technique Long PCR High Yield GC rich templates Unpurified Templates Low copy templates High Specificity RNA templates TA Cloning High Fidelity
Standard PCR   +           +  
Hot Start PCR   +     + +   +  
High Fidelity & Long PCR +   +           +
RT PCR         + + +    

 

Cloning-related Products and Enzymes Selection Guide
Product group Product Cloning Related Applications Main Features
Nucleotides dNTP Mixes All PCR applications Exceptionally pure dNTPs for all demanding PCR applications
dNTP Sets All PCR applications Exceptionally pure dNTPs for all demanding PCR applications
dNTP/dUTP Mixes and Sets PCR carry-over contamination prevention Exceptionally pure dNTPs for all demanding PCR applications
DNA Electrophoresis 1 kb DNA Ladder Sizing and quantification of DNA fragments on the gel 250 bp- 10000 bp rangeReady to use, Sharp bands
100 bp DNA Ladder Sizing and quantification of DNA fragments on the gel 100 bp- 3000 bp rangeReady to use, Sharp bands
50 bp DNA Ladder Sizing and quantification of DNA fragments on the gel 50 bp- 1500 bp range
Ready to use, Sharp bands
DNA Loading Dye, 6x Preparation of  PCR products for loading on the gel Free from nucleases
Molecular Biology Enzymes phi 29 DNA Polymerase Isothermal DNA amplification  Highest processivity, high yields, high accuracy
StranDisplaceTM Thermostable DNA Polymerase Loop mediated isothermal amplification High strand displacement activity
T4 DNA Ligase, Rapid Ligation, cloning of PCR products Fast and efficient ligation
Uracil-DNA Glycosylase Prevention of carry over contamination in PCR Exceptionally pure UDG