Standard PCR typically has the following parameters:
- Target length is 1–3 kb
- GC content of the template is 40–60%
- Template is pure, abundant and unproblematic
- Very high-fidelity of amplification is not required
Unmodified Taq DNA polymerase is the most common enzyme for standard PCR. For simplified reaction setup, PCR master mixes which contain all necessary PCR reagents are offered. Green colored, high-density PCR buffer allows direct loading of PCR products onto gels.
Standard PCR Product Selection Guide
|Product||Main feature||Simplified set-up||Optimization freedom||Direct gel loading||Colored Enzyme|
|Taq DNA Polymerase, recombinant, 5 u/µl||MgCl2 supplied in a separate vial for maximum flexibility||+|
|Taq DNA Polymerase, convenient, 5 u/µl||Optimal concentration of MgCl2 in the Reaction Buffer||+|
|PCR Master Mix, 2x||Premixed PCR reagents: just add template, primers and water||+|
|Green Taq DNA Polymerase, 5 u/µl||Green Reaction Buffer for direct loading on gels after the PCR||+||+|
|Green PCR Master Mix, 2x||Premixed PCR reagents: just add template, primers and water. Green Reaction Buffer for direct loading on gels after the PCR||+||+||+|
NOTE: All above enzymes ensure the same PCR Fidelity like Taq DNA Polymerase, produce PCR products of 3- 5 kb
with A overhangs suitable for TA cloning.