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GenUP™ Virus DNA/RNA Kit

Features

  • Universal kit for simultaneous RNA and DNA isolation from different starting materials
  • Fast and simple procedure with sample-specific protocols
  • High yields of pure RNA and DNA
  • No DNase treatment, no toxic β-mercaptoethanol

Applications

  • Simultaneous viral DNA and RNA isolation from various sources
  • Excellent performance for unknown viruses

Purchase GenUP™ Virus DNA/RNA Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0701101 10 preps Solutions, Mini Filters and Vials
€66.00
BR0701102 50 preps Solutions, Mini Filters and Vials
€218.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit™ GenUP Virus DNA/RNA Kit has been specially developed for quick and easy isolation of viral RNA and DNA. The kit is especially useful when the origin of the virus is unknown. Viral double-stranded DNA and single-stranded RNA are simultaneously isolated from eukaryotic samples, including plasma, serum, and other body fluids as well as cell cultures, tissues, and buccal swabs.

The unique binding membrane of our high-capacity Mini Filters guaranties high yields. A high concentration of purified nucleic acid can be achieved with flexible elution volumes. The kit includes carrier RNA.

After a few initial procedures, the viral nucleic acids are bound to a Mini Filter, washed and then eluted in a separate tube. The purified nucleic acids are ready to be used in all demanding molecular biology applications, including cDNA synthesis, northern blot analysis, qPCR and RT-PCR.


STARTING MATERIAL

Eukaryotic cells (up to 5 × 106)
Serum, plasma, cell-free body fluids, cell culture supernatants (150 µl)
Tissue samples, biopsies (up to 20 mg)
Paraffin-embedded tissues, buccal swabs

EXTRACTION TIME

Approximately 25 min

TYPICAL YIELD

Yield is highly dependent on sample type


STEPS

  • Homogenize and lyse the sample material.
  • Centrifuge to pellet debris.
  • Add Buffer BINDING BD and transfer to a Mini Filter (blue).
  • Centrifuge.
  • Add Buffer WASH A and centrifuge.
  • Add Buffer WASH B and centrifuge.
  • Centrifuge once more to remove residual ethanol.
  • Add Water, RNase-free (for ELUTION), incubate and centrifuge.
  • DNA/RNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

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