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High-Fidelity & Long-Range PCR

Pfu DNA Polymerase provides PCR accuracy that is approximately 10 times higher than Taq DNA Polymerase, and is suitable for demanding cloning, sequencing and expression applications.
Long-range PCR kits provide an optimized combination of Taq DNA polymerase and a proofreading DNA polymerase for longer PCR products with higher accuracy than is possible with the Taq enzyme alone. Long-range enzymes amplify templates of up to 30–40 kb with high fidelity. PCR Enhancer is supplied for better results with GC-rich amplicons. High-fidelity and long-range products are not recommended for use with dUTP.

Optimized — excellent enzyme performance for enhanced applications
Accurate — high-fidelity polymerase
Reliable — polymerase for large amplicons
Fast — master mixes for less pipetting during setup

 

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  1. Pfu DNA Polymerase, 2.5 U/µl

    Pfu DNA Polymerase, 2.5 U/µl

    Features
    • Accurate PCR for demanding applications
    • Approximately ten times higher accuracy than Taq DNA Polymerase
    • Proof-reading for increased fidelity
    Applications
    • High-fidelity PCR
    • Generation of PCR products for blunt cloning
    • Site directed mutagenesis
    Learn More…

    Package Sizes
    Starting at:
    €69.00

    Does not include applicable tax, shipping & handling, or other charges.

  2. 2X Pfu PCR Master Mix

    2X Pfu PCR Master Mix

    Features
    • Optimized Pfu PCR Master Mix for minimal hands-on and fast setup
    • Pure Pfu DNA Polymerase and highest quality dNTPs
    • Approximately ten times higher accuracy than Taq DNA Polymerase for accurate PCR in demanding applications
    Applications
    • High-throughput, high-fidelity PCR
    • Generation of PCR products for blunt cloning
    Learn More…

    Package Sizes
    Starting at:
    €125.00

    Does not include applicable tax, shipping & handling, or other charges.

  3. Long Range DNA Polymerase, 2.5 U/µl

    Long Range DNA Polymerase, 2.5 U/µl

    Features
    • High-productivity, long-range PCR
    • Increased fidelity for accurate amplification of GC-rich templates
    • Polymerase mix for high yield and short cycle times
    Applications
    • Long-range PCR up to 40 kb
    • Amplification of GC-rich templates
    Learn More…

    Package Sizes
    Starting at:
    €69.00

    Does not include applicable tax, shipping & handling, or other charges.

  4. Long Range PCR Master Mix, 2×

    Long Range PCR Master Mix, 2×

    Features
    • Optimized Long Range PCR Master Mix for minimal hands-on and fast setup
    • Mix of pure polymerases and highest quality dNTPs for high yield and short cycle times
    • Increased fidelity for accurate amplification of GC-rich templates
    Applications
    • High-throughput, long-range PCR up to 40 kb
    • Amplification of GC-rich templates
    Learn More…

    Package Sizes
    Starting at:
    €125.00

    Does not include applicable tax, shipping & handling, or other charges.

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