Shopping Cart - €0.00

You have no items in your shopping cart.

Quick Order
Return to Previous Page

GenUP™ Plasmid Kit


  • Universal kit for both high- and low-copy-number plasmids isolation
  • Fast and simple procedure
  • High yields of pure plasmid DNA suitable for all applications


  • Plasmid DNA isolation from Gram-negative and Gram-positive bacteria

Purchase GenUP™ Plasmid Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0700201 10 preps Solutions, Mini Filters and Vials
BR0700202 50 preps Solutions, Mini Filters and Vials
BR0700203 250 preps Solutions, Mini Filters and Vials

*Does not include applicable tax, shipping & handling, or other charges.


biotechrabbit™ GenUP Plasmid Kit is designed for fast and efficient plasmid purification from 0.5–15 ml bacterial suspensions in a mini format. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, plasmid DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. Typically, yields are up to 60 µg plasmid DNA from a 15 ml bacterial suspension, depending on a plasmid copy number.

The purified plasmid DNA is ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.


Bacterial cultures: 0.5–15 ml for high-copy-number plasmids, low-copy-number plasmids or cosmids


Approximately 15 min


Approximately 80 µg plasmid DNA


Variable: 45–60 µg high-copy plasmid DNA is typically purified from 15 ml culture, or 6–20 µg from 2 ml culture 


A260/A280 1.7–2.0


  • Pellet bacterial culture and centrifuge. Remove the supernatant.
  • Resuspend the cell pellet in Buffer RESUSPENSION and vortex.
  • Optional: Pre-treatment of Gram-positive bacteria with lysozyme.
  • Add Buffer LYSIS LP, mix and incubate.
  • Add Buffer NEUTRALIZATION, mix and centrifuge.
  •  Transfer lysate to Mini Filter (orange) and centrifuge.
  • Wash with Buffer WASH PA and Buffer WASH PB and centrifuge.
  • Centrifuge again to remove residual ethanol.
  • Elute DNA with Buffer ELUTION EP.
  • Purified DNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

You may also be interested in the following product(s)
RTS Amino Acid Sampler

RTS Amino Acid Sampler

Package Sizes
Starting at:

Heat Labile Uracil-DNA Glycosylase, 1 U/µl

Heat Labile Uracil-DNA Glycosylase, 1 U/µl

Package Sizes
Starting at:

Purified supernatant antibodies

Purified supernatant antibodies

Package Sizes
Starting at:

ApStarTaq™ DNA Polymerase, 5 U/µl

ApStarTaq™ DNA Polymerase, 5 U/µl

Package Sizes
Starting at:

GenUP™ Plant DNA Kit

GenUP™ Plant DNA Kit

Package Sizes
Starting at: