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GenUP™ Plasmid Kit

Features

  • Universal kit for both high- and low-copy-number plasmids isolation
  • Fast and simple procedure
  • High yields of pure plasmid DNA suitable for all applications

Applications

  • Plasmid DNA isolation from Gram-negative and Gram-positive bacteria

Purchase GenUP™ Plasmid Kit

Availability: In stock

Catalog # Size Package Content Price* Qty
BR0700201 10 preps Solutions, Mini Filters and Vials
€24.00
BR0700202 50 preps Solutions, Mini Filters and Vials
€77.00
BR0700203 250 preps Solutions, Mini Filters and Vials
€316.00

*Does not include applicable tax, shipping & handling, or other charges.

OR

biotechrabbit™ GenUP Plasmid Kit is designed for fast and efficient plasmid purification from 0.5–15 ml bacterial suspensions in a mini format. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, plasmid DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. Typically, yields are up to 60 µg plasmid DNA from a 15 ml bacterial suspension, depending on a plasmid copy number.

The purified plasmid DNA is ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.


STARTING MATERIAL

Bacterial cultures: 0.5–15 ml for high-copy-number plasmids, low-copy-number plasmids or cosmids

EXTRACTION TIME

Approximately 15 min

BINDING CAPACITY

Approximately 80 µg plasmid DNA

TYPICAL YIELD

Variable: 45–60 µg high-copy plasmid DNA is typically purified from 15 ml culture, or 6–20 µg from 2 ml culture 

AVERAGE PURITY

A260/A280 1.7–2.0


STEPS

  • Pellet bacterial culture and centrifuge. Remove the supernatant.
  • Resuspend the cell pellet in Buffer RESUSPENSION and vortex.
  • Optional: Pre-treatment of Gram-positive bacteria with lysozyme.
  • Add Buffer LYSIS LP, mix and incubate.
  • Add Buffer NEUTRALIZATION, mix and centrifuge.
  •  Transfer lysate to Mini Filter (orange) and centrifuge.
  • Wash with Buffer WASH PA and Buffer WASH PB and centrifuge.
  • Centrifuge again to remove residual ethanol.
  • Elute DNA with Buffer ELUTION EP.
  • Purified DNA in the Elution Tube is ready for use.

For detailed protocol, please, refer to the product manual (Related Documents).

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