STARTING MATERIAL

Eukaryotic cells (5 × 10⁶)
Fresh or frozen tissue or biopsies (up to 20 mg)
Gram-positive and Gram-negative bacteria (up to 1 × 10⁹)

EXTRACTION TIME

Typically 15–40 minutes

BINDING CAPACITY

100 µg

Yield

Depends on the type and the amount of the starting material

RECOVERY RATE

High rate of recovery for small RNA molecules

STEPS

• Homogenize and lyse the sample material.
• Centrifuge to pellet unlysed material.

• Transfer the lysate to the Mini Filter DNA (blue).
• Centrifuge, and discard the Mini Filter DNA with bound DNA.
• The filtrate contains the RNA, don’t discard!

• Add isopropanol to the filtrate.
• Transfer to a Mini Filter RNA (violet) to bind RNA and centrifuge.

• Add Buffer WASH A and centrifuge.
• Add Buffer WASH B and centrifuge.
• Centrifuge once more to remove residual ethanol.

• Add Water, RNase-free (for ELUTION), incubate and centrifuge.
• microRNA in the Elution Tube is ready for use.