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RPA

biotechrabbit RPA Products

 

What is Recombinase Polymerase Amplification (RPA)?

Recombinase Polymerase Amplification (RPA) is an isothermal DNA and RNA amplification technology. Unlike traditional PCR methods, RPA eliminates the need for thermal cycling, making it faster and more accessible for both field and laboratory applications.

 

Key Features

  • Isothermal Amplification: Reaction at a constant temperature (37–42°C), no need for thermocyclers.
  • Rapid Results: Amplification can be achieved in as little as 10–20 minutes.
  • High Sensitivity: Detects as few as 100 DNA or RNA copies.
  • Versatile Applications: Suitable for research, diagnostics, and point-of-care testing.

 

Comparison: RPA vs. PCR

Feature RPA PCR
Temperature Isothermal (37–42°C) Thermal cycling (typically 95°C, 60°C, 72°C)
Speed Results in 10–20 minutes 1–2 hours
Equipment Minimal (e.g., heating block) Requires thermal cycler
Portability Ideal for field testing Limited portability
Sensitivity High sensitivity, few DNA/RNA copies needed High, but often requires clean samples
Applications Point-of-care, diagnostics, field work Lab-based diagnostics and research

 

Single Enzymes for RPA

Lyo-ready T6 UvsX DNA Recombinase, 5 mg/ml

LYO-ready T4 UvsY Protein, 2 mg/ml

LYO-ready Exonuclease III, 100 U/µl

LYO-ready RB69 Gene32 Protein, 10 mg/ml

LYO-ready T4 Gene32 Protein, 10 mg/ml

 

RPA Master Mixes

2X RPA basic Master Mix, lyophilized

2X RPA exo Master Mix, lyophilized

2X RT-RPA basic Master Mix, lyophilized

2X RT-RPA exo Master Mix, lyophilized

 

Exo vs. Non-Exo Mixes

Exo Master Mixes

    • Enable real-time fluorescence detection for quantitative analysis.
    • Ideal for sensitive applications like pathogen load monitoring.

Non-Exo (Basic) Master Mixes

    • Designed for end-point detection via colorimetric or gel-based methods.
    • Cost-effective for simpler workflows.