Reverse Transcription & RT-PCR
RevertUP Reverse Transcriptase, which is a proprietary modification of the MMuLV reverse transcriptase, provides most efficient cDNA synthesis without RNase H activity, allowing successful synthesis of cDNAs of greater than 14 kb in length.
One-step RT-PCR kits provide optimized reagents, including Hot Start Taq DNA Polymerase, for performing both reverse-transcription and amplification reactions in one tube.
The Moloney-Murine Leukemia Virus (MMuLV) reverse transcriptase is a classic RNA-dependent DNA polymerase. The exceptional purity of the biotechrabbit MMuLV enzyme ensures excellent results in demanding applications. A special RNase Inhibitor prevents RNA templates from degradation by pervasive RNases.
High performance — exceptional purity for demanding applications
Convenient — excellent efficiency at high temperatures
Reliable — RNase Inhibitor for protecting your RNA
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MMuLV Reverse Transcriptase, 200 U/µl
Features
- Pure reverse transcriptase for cDNA synthesis
- High yields of first-strand cDNA
- High value for a fair price
Learn More…Applications
- First-strand cDNA synthesis
- Generation of labeled cDNA
- RNA analysis by primer extension
Package Sizes
Starting at: €65.00Does not include applicable tax, shipping & handling, or other charges.
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RevertUP™ II Reverse Transcriptase, 200 U/µl
Features
- Improved performance for synthesis of long cDNAs (≥ 19 kb)
- Excellent stability at high temperatures up to 60°C
- High sensitivity for cDNA synthesis from few copies of template
Learn More…Applications
- First-strand cDNA synthesis
- Generation of labeled cDNA
- RNA analysis by primer extension
- cDNA library construction
- RT-PCR
Package Sizes
Starting at: €100.00Does not include applicable tax, shipping & handling, or other charges.
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AnyScript™ Reverse Transcriptase, 200 U/µl
Features
- Increased thermostability
- Exceptional performance for longer templates
- Excellent cDNA yield
Learn More…Applications
- First-strand cDNA synthesis
- cDNA library construction
- RT-PCR
Package Sizes
Starting at: €451.00Does not include applicable tax, shipping & handling, or other charges.
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AllScript™ Reverse Transcriptase, 4 U/µl
Features
- Highly specific and sensitive RT-PCR
- Excellent performance in transcription of complex RNA secondary structures
- High yields of cDNA even with targets in low copy number
- RNase H activity specific to RNA hybridized to cDNA for improved 1step PCR
Learn More…Applications
- Standard reverse transcription
- Synthesis of ds cDNA for cloning
- RT-PCR and qRT-PCR
- Rapid amplification of cDNA ends (RACE)
- RNA analysis by primer extension
Package Sizes
Starting at: €198.00Does not include applicable tax, shipping & handling, or other charges.
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1-Step RT-PCR Master Mix
Features
- Efficient thermostable Reverse Transcriptase and RNase Inhibitor providing high cDNA yields
- Unique Hot Start Taq DNA Polymerase in a mix with high-quality dNTPs
- PCR enhancers allowing sensitive low background amplification
Learn More…Applications
- One-step RT-PCR
- Virus detection
- Amplification of GC-rich and complex templates
Package Sizes
Starting at: €184.00Does not include applicable tax, shipping & handling, or other charges.
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cDNA Synthesis Kit
Features
- Highly efficient synthesis of long cDNAs (≥ 19 kb)
- Excellent yields at temperatures up to 55°C
- High sensitivity reverse transcription from low abundance template
- Superior performance in demanding applications, including templates with a high degree of secondary structure
Learn More…Applications
- First-strand cDNA synthesis for RT-PCR and qPCR
- Gene expression profiling
- RNA labeling and primer extension
- cDNA library construction
Package Sizes
Starting at: €64.00Does not include applicable tax, shipping & handling, or other charges.
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RNase Inhibitor, 40 U/µl
Features
- Exceptionally pure proprietary Ribonuclease Inhibitor for demanding RNA applications
- Active under variety of reaction conditions used for work with RNA
- Prevention of RNA from degradation by a wide range of RNases
Learn More…Applications
- In vitro transcription/translation
- cDNA synthesis
- RNA purification and storage
Package Sizes
Starting at: €63.00Does not include applicable tax, shipping & handling, or other charges.
