biotechrabbit™ GenUP Blood RNA Kit is designed for fast isolation of total RNA from up to 1 ml whole blood from fresh or frozen samples that have been stabilized with EDTA or citrate. Erythrocytes are removed in an initial lysis step. After an second lysis step, genomic DNA is bound to a Mini Filter DNA, which can be discarded. RNA is selectively bound to a Mini Filter RNA, washed with two different buffers and eluted. Including lysis, isolated RNA is available in approximately 45 min. The isolated RNA is suitable for a wide range of different molecular biology applications, including RT-PCR.
The GenUP Blood RNA Kit is designed for the use with blood. For other starting material, such as cell-free body fluids (including cerebrospinal fluid, serum, plasma or urine), tissue, stool samples, buffy coat, cultured or isolated cells, swabs, dried blood spots, viruses, fungi, bacteria or parasites, please refer to the GenUP Total RNA Kit (BR07009), GenUP Virus RNA Kit (BR07010), GenUP Virus DNA/RNA Kit (BR07011) or UPzol RNA Isolation Solution (BR07001).
STARTING MATERIAL | Fresh whole blood; stabilized with EDTA or citrate (0.5–1.0 ml) |
EXTRACTION TIME | Approximately 45 min |
BINDING CAPACITY | > 20 µg RNA |
TYPICAL YIELD | Variable depending on the starting material; 1–8 µg RNA |
STEPS |
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For detailed protocol, please, refer to the product manual (Related Documents).
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