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RTS 500 Wheat Germ CECF Kit
Features
- Successful expression of eukaryotic proteins , generally without sequence optimization
- Synthesis of up to 1 mg functional eukaryotic protein
- Maximized production of active heat-sensitive proteins, due to an optimum reaction temperature of 24°C
Applications
- Up to milligram-scale protein expression of functional eukaryotic proteins
- Expression of multiple proteins in parallel
Package Sizes
Starting at: €2,560.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Wheat Germ LinTempGen Set, His6-tag
Features
- Optimized spacing of eukaryotic regulatory elements
- Time saving, no laborious cloning
- Flexible positioning of His6-tag at the C- or N-terminus
- Primary PCR product can be cloned into pIVEX vectors
Applications
- Rapid generation of linear expression constructs for screening applications
- Linear template generation for optimized His6-tagged protein expression
Package Sizes
Starting at: €350.00Does not include applicable tax, shipping & handling, or other charges.
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RTS pIVEX Wheat Germ His6-tag Vector Set
Features
- Stable circular templates ensure high yields in preparative-scale expression reactions
- For construction of C- and N-terminal His6-tag proteins
Applications
- Expression vector for preparative-scale expression in eukaryotic wheat germ lysates
Package Sizes
Starting at: €410.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 100 Insect Membrane Kit
Features
- Robust expression of a wide range of proteins (clotting factors, protein kinases, transcription factors)
- Yields of up to 50 μg/ml protein, sufficient for functional studies or screening procedures
- Compatibility with linear templates and plasmids
- Expression screening in small volumes (>5 μl)
Applications
- Expression of membrane proteins without the need for additives • Synthesis of pos
- Synthesis of post-translationally modified eukaryotic proteins
Package Sizes
Starting at: €235.00Does not include applicable tax, shipping & handling, or other charges.
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RTS DnaK Supplement
Features
- E. coli chaperones to enhance correct folding and increase solubility, even of large proteins
- Compatible with RTS 100, 500, 9000 E. coli kits
Applications
- Enhance the expression of soluble proteins
- Improved synthesis of functionally activity proteins
Package Sizes
Starting at: €350.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 500 Adapter
Features
- Compatible with CECF Devices from the RTS 500 ProteoMaster E. coli Kit, RTS 500 E. coli Disulfide Kit and the RTS 500 Wheat Germ Kit
- Compatible with Eppendorf SmartBlock plates in combination with the Eppendorf ThermoMixer C (also suitable with the legacy Eppendorf ThermoMixer comfort in combination with MTP Thermoblock)
Applications
- Frame for holding up to six RTS 500 CECF devices
Package Sizes
Starting at: €92.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Linear Template Fab Kit
Features
- Introduction of an affinity tag for protein purification
- Signal peptide sequence for translocation into microsomes
- Introduction of a signal peptide sequence for translocation into microsomes
- Compatibility with E. coli HY, Fab and Insect kits
Applications
- PCR-based inear templates for protein expression in E. coli and insect disulfide kits
- Fast template generation for expression of Fab and scFv antibody fragments
Package Sizes
Starting at: €360.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 500 E. coli Disulfide Kit
Features
- Optimized reaction conditions for the expression of functional disulfide-bonded proteins
- No need for refolding aggregated proteins
- CECF technology for yields of up to 2.5 mg
Applications
- Expression of active proteins containing multiple disulfide bonds
Package Sizes
Starting at: €2,560.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Linear Template Kit Plus
Features
- Efficient in vitro expression (E. coli and insect kits)
- Primers for affinity tags (His6-tag /Strep-tag II)
- Signal peptide sequence primers for expression translocation into microsomes (insect systems)
Applications
- Fast generation of multiple template constructs
- Accelerated screening of protein expression templates and conditions
Package Sizes
Starting at: €345.00Does not include applicable tax, shipping & handling, or other charges.
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RTS pIX3.0 Vector
Features
- Multiple cloning site compatible with linear templates generated with RTS Linear Template Kit Plus and RTS Linear Template Fab Kit
- High copy origin of replication
- Cell-free protein synthesis scale-up to after expression screening using linear templates
- Free of any potentially interfering regulatory elements ensuring efficient transcription and translation
- Compatible with RTS E. coli and insect kits
Applications
- Cloning of RTS Linear Templates for expression in RTS E. coli and insect kits
Package Sizes
Starting at: €356.00Does not include applicable tax, shipping & handling, or other charges.