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RTS 100 Insect Membrane Kit
Features
- Robust expression of a wide range of proteins (clotting factors, protein kinases, transcription factors)
- Yields of up to 50 μg/ml protein, sufficient for functional studies or screening procedures
- Compatibility with linear templates and plasmids
- Expression screening in small volumes (>5 μl)
Learn More…Applications
- Expression of membrane proteins without the need for additives • Synthesis of pos
- Synthesis of post-translationally modified eukaryotic proteins
Package Sizes
Starting at: €235.00Does not include applicable tax, shipping & handling, or other charges.
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RTS GroE Supplement
Features
- E. coli chaperone mix to enhance correct folding and increase solubility of expressed proteins
- Compatible with RTS 100, 500, 9000 E. coli platforms
Learn More…Applications
- Improved synthesis of functionally active proteins
Package Sizes
Starting at: €366.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 500 Adapter
Features
- Compatible with CECF Devices from the RTS 500 ProteoMaster E. coli Kit, RTS 500 E. coli Disulfide Kit and the RTS 500 Wheat Germ Kit
- Compatible with Eppendorf SmartBlock plates in combination with the Eppendorf ThermoMixer C (also suitable with the legacy Eppendorf ThermoMixer comfort in combination with MTP Thermoblock)
Learn More…Applications
- Frame for holding up to six RTS 500 CECF devices
Package Sizes
Starting at: €92.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Linear Template Fab Kit
Features
- Introduction of an affinity tag for protein purification
- Signal peptide sequence for translocation into microsomes
- Introduction of a signal peptide sequence for translocation into microsomes
- Compatibility with E. coli HY, Fab and Insect kits
Learn More…Applications
- PCR-based inear templates for protein expression in E. coli and insect disulfide kits
- Fast template generation for expression of Fab and scFv antibody fragments
Package Sizes
Starting at: €360.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Linear Template Kit Plus
Features
- Efficient in vitro expression (E. coli and insect kits)
- Primers for affinity tags (His6-tag /Strep-tag II)
- Signal peptide sequence primers for expression translocation into microsomes (insect systems)
Learn More…Applications
- Fast generation of multiple template constructs
- Accelerated screening of protein expression templates and conditions
Package Sizes
Starting at: €345.00Does not include applicable tax, shipping & handling, or other charges.
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RTS pIX3.0 Vector
Features
- Multiple cloning site compatible with linear templates generated with RTS Linear Template Kit Plus and RTS Linear Template Fab Kit
- High copy origin of replication
- Cell-free protein synthesis scale-up to after expression screening using linear templates
- Free of any potentially interfering regulatory elements ensuring efficient transcription and translation
- Compatible with RTS E. coli and insect kits
Learn More…Applications
- Cloning of RTS Linear Templates for expression in RTS E. coli and insect kits
Package Sizes
Starting at: €356.00Does not include applicable tax, shipping & handling, or other charges.
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cDNA Synthesis Kit
Features
- Highly efficient synthesis of long cDNAs (≥ 19 kb)
- Excellent yields at temperatures up to 55°C
- High sensitivity reverse transcription from low abundance template
- Superior performance in demanding applications, including templates with a high degree of secondary structure
Learn More…Applications
- First-strand cDNA synthesis for RT-PCR and qPCR
- Gene expression profiling
- RNA labeling and primer extension
- cDNA library construction
Package Sizes
Starting at: €64.00Does not include applicable tax, shipping & handling, or other charges.
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RevertUP™ II Reverse Transcriptase, 200 U/µl
Features
- Improved performance for synthesis of long cDNAs (≥ 19 kb)
- Excellent stability at high temperatures up to 60°C
- High sensitivity for cDNA synthesis from few copies of template
Learn More…Applications
- First-strand cDNA synthesis
- Generation of labeled cDNA
- RNA analysis by primer extension
- cDNA library construction
- RT-PCR
Package Sizes
Starting at: €100.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 100 Wheat Germ Kit
Features
- Efficient expression of up to 5 μg protein per reaction
- Compatible with non-optimized, pro- and eukaryotic T7 promoter-based vectors as well as linear templates
- Excellent homogeneity of large proteins (264 kDa expressed)
- No endotoxins, allows direct use in cell-based assays
- Upscaling using high yield CECF wheat germ kits
Learn More…Applications
- Fast parallel synthesis for screening purposes
- Synthesis of mammalian or other proteins for functional testing without the disturbing endogenous protein background that is typical for in vivo expression
- Synthesis of proteins dependent on eukaryotic chaperones
Package Sizes
Starting at: €740.00Does not include applicable tax, shipping & handling, or other charges.
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T7 RNA Polymerase, 50 U/µl
Features
- High-yield in vitro transcription
- Strictly promoter-specific activity
- No promoter cross-talk
- Free from endonuclease, exonuclease, protease, RNase
Learn More…Applications
- Preparation of homogeneously labeled RNA probes for hybridization (e.g., radioactive, biotin, DIG-11-UTP)
- Generation of mRNA for in vitro translation, gene expression or splicing studies
- Production of template for cDNA synthesis
- In vitro synthesis of capped RNA transcripts
- Synthesis of antisense RNA and dsRNA for RNA interference or gene silencing
Package Sizes
Starting at: €60.00Does not include applicable tax, shipping & handling, or other charges.
