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2X Hot-Start PCR Master Mix, lyophilized
Features
- Room-temperature stable enzymes and mixes
- Exceptionally pure Taq DNA Polymerase
- Hot-start for high PCR specificity and sensitivity
- Optimized Master Mix for fast setup
Learn More…Applications
- Ambient shipment and room-temperature storage
- Routine and demanding PCR applications
- PCR amplification up to 3 kb
- TA cloning
Package Sizes
Starting at: €149.00Does not include applicable tax, shipping & handling, or other charges.
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T7 RNA Polymerase, 50 U/µl
Features
- High-yield in vitro transcription
- Strictly promoter-specific activity
- No promoter cross-talk
- Free from endonuclease, exonuclease, protease, RNase
Learn More…Applications
- Preparation of homogeneously labeled RNA probes for hybridization (e.g., radioactive, biotin, DIG-11-UTP)
- Generation of mRNA for in vitro translation, gene expression or splicing studies
- Production of template for cDNA synthesis
- In vitro synthesis of capped RNA transcripts
- Synthesis of antisense RNA and dsRNA for RNA interference or gene silencing
Package Sizes
Starting at: €60.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 100 Wheat Germ Kit
Features
- Efficient expression of up to 5 μg protein per reaction
- Compatible with non-optimized, pro- and eukaryotic T7 promoter-based vectors as well as linear templates
- Excellent homogeneity of large proteins (264 kDa expressed)
- No endotoxins, allows direct use in cell-based assays
- Upscaling using high yield CECF wheat germ kits
Learn More…Applications
- Fast parallel synthesis for screening purposes
- Synthesis of mammalian or other proteins for functional testing without the disturbing endogenous protein background that is typical for in vivo expression
- Synthesis of proteins dependent on eukaryotic chaperones
Package Sizes
Starting at: €740.00Does not include applicable tax, shipping & handling, or other charges.
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2X Multiplex Hot-Start PCR Master Mix
Features
- Excellent performance and robustness in multiplex PCR
- Optimized Master Mix for minimal hands-on and fast setup
- Hot-start for highest sensitivity and specificity
- Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs
Learn More…Applications
- Fast and high-throughput multiplex PCR
- Parallel detection of multiple targets in a single assay
- Gene expression analysis, diagnostic and forensic genotyping
- Amplification of 50 bp to 2 kb targets
Package Sizes
Starting at: €60.00Does not include applicable tax, shipping & handling, or other charges.
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cDNA Synthesis Kit
Features
- Highly efficient synthesis of long cDNAs (≥ 19 kb)
- Excellent yields at temperatures up to 55°C
- High sensitivity reverse transcription from low abundance template
- Superior performance in demanding applications, including templates with a high degree of secondary structure
Learn More…Applications
- First-strand cDNA synthesis for RT-PCR and qPCR
- Gene expression profiling
- RNA labeling and primer extension
- cDNA library construction
Package Sizes
Starting at: €64.00Does not include applicable tax, shipping & handling, or other charges.
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Tris-NTA Biotin
Features
- A complex of three Ni-NTA groups ensures high-affinity binding of His-tags
- Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators
- Protein binding is stoichiometric
Learn More…Applications
- Reversible labeling of proteins or cell surfaces
- Detection and analysis of target molecules
- Immobilization of proteins, lipids and cells on surfaces
- Purification and sample preparation of proteins
- Coupling with microscopic or spectroscopic probes
Package Sizes
Starting at: €360.00Does not include applicable tax, shipping & handling, or other charges.
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Tris-NTA Amine
Features
- A complex of three Ni-NTA groups ensures high-affinity binding of His-tags
- Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators
- Protein binding is stoichiometric
Learn More…Applications
- Reversible labeling of proteins or cell surfaces
- Detection and analysis of target molecules
- Immobilization of proteins, lipids and cells on surfaces
- Purification and sample preparation of proteins
- Coupling with microscopic or spectroscopic probes
Package Sizes
Starting at: €215.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Linear Template Kit Plus
Features
- Efficient in vitro expression (E. coli and insect kits)
- Primers for affinity tags (His6-tag /Strep-tag II)
- Signal peptide sequence primers for expression translocation into microsomes (insect systems)
Learn More…Applications
- Fast generation of multiple template constructs
- Accelerated screening of protein expression templates and conditions
Package Sizes
Starting at: €345.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 500 E. coli Disulfide Kit
Features
- Optimized reaction conditions for the expression of functional disulfide-bonded proteins
- No need for refolding aggregated proteins
- CECF technology for yields of up to 2.5 mg
Learn More…Applications
- Expression of active proteins containing multiple disulfide bonds
Package Sizes
Starting at: €2,560.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Linear Template Fab Kit
Features
- Introduction of an affinity tag for protein purification
- Signal peptide sequence for translocation into microsomes
- Introduction of a signal peptide sequence for translocation into microsomes
- Compatibility with E. coli HY, Fab and Insect kits
Learn More…Applications
- PCR-based inear templates for protein expression in E. coli and insect disulfide kits
- Fast template generation for expression of Fab and scFv antibody fragments
Package Sizes
Starting at: €360.00Does not include applicable tax, shipping & handling, or other charges.
